Flashcards in 9-3 Niesseria/Hemophillus/Bordetella Deck (19)
What 3 things does the [Polysaccharide capsule] likely provide for Neisseria?
1. bloodstream invasion
3. possibly CNS penetration
A: What 2 components of Neisseria generate CNS inflammation/Local Inflammation?
B: What 2 HOST chemicals mediate this?
CNS/Local inflammation generated by
1. [cell wall peptidoglycan]
B: mediated by IL-1 and TNF
4 Clinical Manifestations of [Neisseria MENINGITIDIS]
1. Respiratory colonization followed by overt disease or transient carrier state
3. Skin: petechiae → purpura
4. Meningococcemia: shock, hemorrhage and purpura,
destruction of adrenals
A: Describe the [Neisseria Meningitidis] Vaccine
A2: What part of the immune system does it stimulate
B: Who is it for? (3)
A: Polysaccharides from Group A, C, Y , W-135 conjugated to conjugated to either diphtheria toxoid, [N. meningitides OMP] or [tetanus toxoid]
A2: T-cell dependent antigens
-Starts at 2 months y/o
-[all adolescents age 11-12]
-[at risk adults]
Define these [NEISSERIA GONORRHEAE] structures:
A: PorB - Outer membrane protein 1 (Porin) that acts as a channel and facilitates epithelial cell invasion
B: Opa - adherence protein tht confer OPAQUE appearance to colony
4. Rmp (Reduction Modifiable Protein) – stimulates [blocking antibodies] to literally block the good antibodies
What is the difference between NEISSERIA GONORRHEAE [Antigenic variation] vs. [Phase variation]
1. Pili - Antigenic variation
a. recombination by transfer of pilS to the expression locus, pilE
2. Opa – Phase variation
a. Can switch on and off as many as 11 different Opa
genes throughout the genome
A: List the attachment sites for
B: How does [Neisseria Gonorrhea] obtain iron
1. Pilli attach to
-[nonciliated fallopian tube epithelium]
2. Opa attach to
B: Has ability to bind to host cell transferrin and obtain iron
A: Disseminated [Neisseria gonococcal] infection can lead to what 3 things?
B: Incubation time for [Neisseria gonococcal]
C: Who is [Gram Stain] testing more sensitive for? [Male vs. Female]
Disseminated gonococcal infection – 1-3% women>men
c. Endocarditis, meningitis – rare
B: 2-5 Days
C: MALES have more sensitive/accurate [Gram Stain Test]
What are the two Penicillin resistance types
Penicillin resistance - two types
a. decreased affinity for penicillin of penicillin-binding proteins
b. Plasmid mediated TEM-type β-lactamase (PPNG)
Which [Haemophilus Influenzae] biogroup causes [Brazilian Purpuric Fever]?
B: Describe [Brazilian Purpuric Fever] (4)
H. influenzae biogroup AEGYPTIUS causes Brazilian purpuric fever
B: HIGHLY LETHAL Conjunctivitis which may progress to
Major virulence factor for [Haemophilus Influenzae]
TYPE B - polyribitol phosphate (PRP)
-Also contained in Vaccines to DEC childhood invasive infection and meningitis
NON-encapsulated [Haemophilus influenzae] commonly colonize the _______, rarely cause _______, but are common causes of ____(3)
Unencapsulated H. influenzae commonly colonize the upper airway, rarely cause invasive disease, but are common causes of
-exacerbations of chronic bronchitis
A: Explain Cellulitis caused by [Haemophilus influenzae]
B: What age group does it occur in
A: Purplish, erythematous swelling involving cheek, periorbital area
B:children 2-4 years
A: Which [Haemophilus Influenzae] causes an [STD Chancroid]?
B: What is the main sx
C: Why is this a problem for future Dz?
D: Which Factor does this strain need to grow in Chocolate Agar?
A: [STD Chancroid] - [Haemophilus ducreyi] .
B: painful genital ulcer w/ lymphadenopathy.
C: Open sores enhance risk of transmission of HIV
D: Factor 5
A: How does the [A subunit] of [Pertussis Toxin] work molecularly?
B: What are the 3 results of this
C: How does the [B subunit] of [Pertussis Toxin] work molecularly
A: [A subunit] catalyzes the ADP ribosylation of a
cellular regulatory protein (G- protein) ---> prevents inactivation of the [adenylate cyclase]---> INC cAMP
ºIncreased respiratory secretions --> Cough
ºmucus production --> Cough
C: [B subunits] bind to receptors on ciliated respiratory cells and phagocytic cells
What 2 things does the [Adenylate cyclase PERTUSSIS TOXIN] do?
Adenylate cyclase toxin
a) Causes increased cAMP levels in host cells
b) Interferes with chemotaxis and superoxide production
List the 5 [Bordatella Pertussis] Toxins
B: Which one causes IL1 release and fever?
1. Pertussis Toxin
2. [Adenylate cyclase] Toxin
3. Tracheal cytotoxin = causes extrusion of [ciliated rep epithelial cells] AND [IL1 release / Fever]
4. Dermonecrotic Toxin
5. Endotoxin (AKA LPS)
1. Incubation time for [Bordatella Pertussis]
2. What sx are in the Catarrhal Stage? (4)
3. describe the Cough (5)
4. What is the [Convalescent stage]
1. Incubation 7-10 days
2. Catarrhal stage - rhinorrhea, fever, sneezing, anorexia
3. Paroxysmal cough:
-up to 50 times a day for 2-4 weeks.
4. Convalescent stage - COUGH gradually fades but can last for 100 days