9-3 Niesseria/Hemophillus/Bordetella Flashcards Preview

M2 PATHO EXAM 3 > 9-3 Niesseria/Hemophillus/Bordetella > Flashcards

Flashcards in 9-3 Niesseria/Hemophillus/Bordetella Deck (19)
Loading flashcards...
1

What 3 things does the [Polysaccharide capsule] likely provide for Neisseria?

1. bloodstream invasion
2. survival
3. possibly CNS penetration

2

A: What 2 components of Neisseria generate CNS inflammation/Local Inflammation?

B: What 2 HOST chemicals mediate this?

CNS/Local inflammation generated by
1. [cell wall peptidoglycan]
2. LPS

B: mediated by IL-1 and TNF

3

4 Clinical Manifestations of [Neisseria MENINGITIDIS]

1. Respiratory colonization followed by overt disease or transient carrier state

2. Meningitis

3. Skin: petechiae → purpura

4. Meningococcemia: shock, hemorrhage and purpura,
destruction of adrenals

4

A: Describe the [Neisseria Meningitidis] Vaccine

A2: What part of the immune system does it stimulate

B: Who is it for? (3)

A: Polysaccharides from Group A, C, Y , W-135 conjugated to conjugated to either diphtheria toxoid, [N. meningitides OMP] or [tetanus toxoid]

A2: T-cell dependent antigens

B:
-Starts at 2 months y/o
-[all adolescents age 11-12]
-[at risk adults]

5

Define these [NEISSERIA GONORRHEAE] structures:

A: PorB

B: Opa

C: Rmp

A: PorB - Outer membrane protein 1 (Porin) that acts as a channel and facilitates epithelial cell invasion

B: Opa - adherence protein tht confer OPAQUE appearance to colony

4. Rmp (Reduction Modifiable Protein) – stimulates [blocking antibodies] to literally block the good antibodies

6

What is the difference between NEISSERIA GONORRHEAE [Antigenic variation] vs. [Phase variation]

1. Pili - Antigenic variation
a. recombination by transfer of pilS to the expression locus, pilE

vs.

2. Opa – Phase variation
a. Can switch on and off as many as 11 different Opa
genes throughout the genome

7

A: List the attachment sites for
*Pilli (5)

*Opa (2)

B: How does [Neisseria Gonorrhea] obtain iron

1. Pilli attach to
-urethra
-[vaginal epithelium]
-[nonciliated fallopian tube epithelium]
-neutrophils
-sperm

2. Opa attach to
-urethra
-[cervical epithelium]

B: Has ability to bind to host cell transferrin and obtain iron

8

A: Disseminated [Neisseria gonococcal] infection can lead to what 3 things?

B: Incubation time for [Neisseria gonococcal]

C: Who is [Gram Stain] testing more sensitive for? [Male vs. Female]

Disseminated gonococcal infection – 1-3% women>men
a. Arthritis
b. Dermatitis
c. Endocarditis, meningitis – rare

B: 2-5 Days

C: MALES have more sensitive/accurate [Gram Stain Test]

9

What are the two Penicillin resistance types

Penicillin resistance - two types
a. decreased affinity for penicillin of penicillin-binding proteins
b. Plasmid mediated TEM-type β-lactamase (PPNG)

10

Which [Haemophilus Influenzae] biogroup causes [Brazilian Purpuric Fever]?

B: Describe [Brazilian Purpuric Fever] (4)

H. influenzae biogroup AEGYPTIUS causes Brazilian purpuric fever

B: HIGHLY LETHAL Conjunctivitis which may progress to
-fever,
- petechiae,
-purpura
-shock

11

Major virulence factor for [Haemophilus Influenzae]

TYPE B - polyribitol phosphate (PRP)

-Also contained in Vaccines to DEC childhood invasive infection and meningitis

12

NON-encapsulated [Haemophilus influenzae] commonly colonize the _______, rarely cause _______, but are common causes of ____(3)

Unencapsulated H. influenzae commonly colonize the upper airway, rarely cause invasive disease, but are common causes of
-sinusitis,
-otitis media
-exacerbations of chronic bronchitis

13

A: Explain Cellulitis caused by [Haemophilus influenzae]

B: What age group does it occur in

A: Purplish, erythematous swelling involving cheek, periorbital area

B:children 2-4 years

14

A: Which [Haemophilus Influenzae] causes an [STD Chancroid]?

B: What is the main sx

C: Why is this a problem for future Dz?

D: Which Factor does this strain need to grow in Chocolate Agar?

A: [STD Chancroid] - [Haemophilus ducreyi] .

B: painful genital ulcer w/ lymphadenopathy.

C: Open sores enhance risk of transmission of HIV

D: Factor 5

15

A: How does the [A subunit] of [Pertussis Toxin] work molecularly?

B: What are the 3 results of this

C: How does the [B subunit] of [Pertussis Toxin] work molecularly

A: [A subunit] catalyzes the ADP ribosylation of a
cellular regulatory protein (G- protein) ---> prevents inactivation of the [adenylate cyclase]---> INC cAMP

B:
ºIncreased respiratory secretions --> Cough
ºmucus production --> Cough
ºlymphocytosis

C: [B subunits] bind to receptors on ciliated respiratory cells and phagocytic cells

16

What 2 things does the [Adenylate cyclase PERTUSSIS TOXIN] do?

Adenylate cyclase toxin
a) Causes increased cAMP levels in host cells
b) Interferes with chemotaxis and superoxide production
of leukocytes

17

List the 5 [Bordatella Pertussis] Toxins

B: Which one causes IL1 release and fever?

1. Pertussis Toxin
2. [Adenylate cyclase] Toxin

3. Tracheal cytotoxin = causes extrusion of [ciliated rep epithelial cells] AND [IL1 release / Fever]

4. Dermonecrotic Toxin
5. Endotoxin (AKA LPS)

18

1. Incubation time for [Bordatella Pertussis]

2. What sx are in the Catarrhal Stage? (4)

3. describe the Cough (5)

4. What is the [Convalescent stage]

1. Incubation 7-10 days
2. Catarrhal stage - rhinorrhea, fever, sneezing, anorexia

3. Paroxysmal cough:
-up to 50 times a day for 2-4 weeks.
-Inspiratory whoop,
-vomiting,
-mucoid secretions,
-[marked lymphocytosis]

4. Convalescent stage - COUGH gradually fades but can last for 100 days

19

A: Recommended [Bordatella Pertussis] Vaccine Regimen for Adults

B: What 3 components is the [acellular Bordatella Pertussis Vaccine] composed of?

A: TDaP, is recommended for all adults (every 10 years)

B:
1. detoxified pertussis toxin (PT),
2. filamentous hemagglutinin (Fha),
3. Pertactin (Pn)