Adenoviridae Flashcards
(33 cards)
What are the two subfamilies of Adenoviridae?
1) Mastadenovirus: Mammals
2) Aviadenovirus: Birds
Describe the morphology of an adenovirus. What is the difference between the penton fibers of the subfamilies?
- hexagon, non-enveloped, icosahedral
- 12 fibers, one at each vertex, protruding from capsid
- Capsid is 720 hexagon subunits arranged as 240 trimers
- Mastadenovirus: single penton at each vertex
- Aviadenovirus : each penton fiber is bifurcated
How do adenoviruses agglutinate RBCs?
tips of the penton fibers bind to the RBC surface
Adenoviruses are stable but…
easily inactivated by disinfectants
Which aspects of the adenovirus are especially toxic to the host cell?
penton and fiber proteins
What type of pathogenesis do adenoviruses cause?
acute respiratory or GI disease (but MOSTLY subclinical)
How are adenoviruses involved in immunosuppression?
- encode proteins that suppress the host immune and inflammatory responses
- inhibits MHC I transport
- inhibits TNF-induced apoptosis
- blocks IFN that inhibits viral protein synthesis
- inhibits kB –> decreases the inflammatory response
T/F. Adenoviruses exhibit long periods of latency.
True
What is the latency site of adenoviruses?
Lymphoid tissue
What are the functions of E1A and E1B in adenovirus oncogenesis?
E1A: inactivates Rb protein
E1B: inactivates p53 protein
What are the disease names for Canine Adenovirus 1?
Infectious Canine Hepatitis
Rubarth’s Disease
Why were ferrets important in distinguishing infectious canine hepatitis from canine distemper?
Ferrets are resistant to ICH but susceptible to CD
Describe transmission of CAV1
- acute infection: in ALL secretions and excretions
- shed in urine 6-9 months (dogs that recover)
- ORONASAL: contact with infected dog secretions/excretions, fomites, ectoparasites with CAV1
What are the target organs for CAV 1 replication?
Liver, kidneys, spleen, lungs
How high should an antibody titer be for a dog to exhibit little clinical signs associated with CAV1?
over 500
What happens in a dog that is infected with CAV1 and has an antibody titer less than 4?
hepatic necrosis
Describe CAV1 inclusion bodies
basophilic intranuclear
T/F. Chronic kidney lesions may result from CAV1.
True
Describe the pathogenesis of Blue Eye associated with CAV1
- corneal edema
- viremia, enters eye via uveal tract –> replicates in corneal endothelium –> viral AB immune complexes –> immune response causes damage to corneal endothelium –> aqueous humor enters cornea –> edematous fluid in corneal stroma
By day ____, severe anterior uveitis and blue eye develop.
7
Which dogs are most susceptible to blue eye?
Dogs in recovery or in chronic cases
T/F. Jaundice is common in an early acute infection of CAV1.
False. NOT common in early acute infection
T/F. Most CAV1 infections are asymptomatic.
True. But can vary from fever to death
What is a characteristic necropsy finding associated with CAV1?
paint brush hemorrhages in the gastric serosa, LNs, thymus, pancreas, subQ
