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Parasitology LAB > AMOEBA > Flashcards

AMOEBA Flashcards

1
Q

Amoebas

A

Entamoeba coli

Endolimax nana

Iodamoeba buetschlii

Entamoeba gingivialis

Entamoeba histolvtica

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2
Q

Trophozoite Stage (I & H preparation)

Shape: condensed, thickened, rounded
mass size: 15 - 50 micra

A

Entamoeba coli

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3
Q

Trophozoite

cytoplasm is grayish violet; there is no clear demarcation between the ectoplasm and endoplasm

the endoplasm is highly vacuolated containing bacteria

the nucleus is spherical with thick nuclear membrane lined with coarse irregular chromatin granules or plaques; the karyosome is moderately large and eccentrically located

A

Entamoeba coli

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4
Q

Cystic Stage

shape: spherical
size: 10 - 33 micra

A

Entamoeba coli

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5
Q

Cyst

smooth, definite, thick cystic wall

number of nuclei range from 1 - 8 rarely more with an eccentric karyosome

in younger cysts, chromatoidal bars with splintered ends and glycogen mass may be present

A

Entamoeba coli

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6
Q

1 & H preparation cytoplasm is granular, grayish violet
nuclei are clearly visible with dark stained eccentric karvosome
• if present, chromatoidal bodies are bluish black
• if present. glycogen mass is unstained

A

Entamoeba coli

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7
Q

NSS preparation
refractile cyst wall cytoplasm is colorless
nuclei are fairly visible as refractile rings
• if present, the chromatoidal bodies are refractile structures
• if present, the glycogen mass is a clear or empty space in the cvioplasm

A

Entamoeba coli

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8
Q

lodine preparation
• cytoplasm is yellowish brewn
• nuclei are clearly seen with eccentric karyosome
• if present, chromatoidal bodies are refractile
• if present, glycogen mass is dark brown in color

A

Entamoeba coli

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9
Q

Trophozoite Stage (I & H preparation)

shape: irregular
• size: small, 6 - 15 micra

A

Endolimax nana

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10
Q

Trophozoite

cytoplasm is grayish-violet, vacuolated which may contain bacteria

there is small, spherical, single nucleus with a large irregular, central or eccentric karyosome

A

Endolimax nana

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11
Q

Cystic Stage

shape: oval
• size: 6 - 10 micra

A

Endolimax nana

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12
Q

Cyst

smooth, definite cystic wall number of

nuclei ranges from 1-4

A

Endolimax nana

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13
Q
  • I & H preparation

cytoplasm is grayish, granular

nuclei are visible as darkly stained dots

chromatoidal bodies occasionally seen as deeply stained, small, slightly curved rods

A

Endolimax nana

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14
Q

NSS preparation

refractile cyst wall

cytoplasm is colorless;

nuclei are faintly visible

A

Endolimax nana

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15
Q

lodine preparation

cytoplasm is yellowish

nuclei are visible as dots

glycogen mass is usually absent; if present, it is usually diffused with ill-
defined margins

A

Endolimax nana

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16
Q

Trophozoite Stage (I & H preparation)

shape: irregular
shape: 6 - 25 micra

A

Iodamoeba buetschlii

17
Q

Trophozoite

granular, vacuolated cytoplasm and vacuoles may contain bacteria and yeast cells

single, spherical nucleus with a large central or eccentric karyosome surrounded by an unstained halo

A

Iodamoeba buetschlii

18
Q

Cystic Stage
Shape: irregular
size: 6 - 15 micra

A

Iodamoeba buetschlii

19
Q

Cyst

smooth, definite cystic wall

have a single nucleus, rarely with 2 nuclei

large glycogen vacuole

A

Iodamoeba buetschlii

20
Q

1& H preparation
• vio et-gray granular cytoplasm

nucleus is visible

large glycogen vacuole is unstained
• chromatoidal bodies are usually absent; if present, they are seen as deeply stained small rods

A

Iodamoeba buetschlii

21
Q

NSS preparation
• retractile, relatively thick cyst wall cytoplasm is colorless
• nuclei is not visible
glycogen vacuole is unstained or an empty space in the cytoplasm

A

Iodamoeba buetschlii

22
Q

lodine preparation

cytoplasm is yellowish

nucleus may be visible

glycogen vacuole is clark brown

A

Iodamoeba buetschlii

23
Q

Trophozoite Stage (I & H preparation)

Shape: irregular
shape: 5 - 35 micra

A

Entamoeba gingivialis

24
Q

Trophozoite

clear demarcation between the clear ectoplasm and granular endoplasm

endoplasm contains food vacuoles with bacteria and rarely RBC

possesses a single nucleus which is spherical in shape, karyosome is near the center

have multiple pseudopodia with progressive directional movement similar to E. histolytica

A

Entamoeba gingivialis

25
Q

Trophozoite Stage (I & H preparation)

shape: irregular
shape: 10–60 micra

A

Entamoeba histolytica

26
Q

Trophozoite

cytoplasm is grayish violet with a clear demarcation between the ectoplasm and endoplasm

endoplasm is briefly granular with ingested bc which are bluish black in color

single, spherical nucleus; nucleus membrane is lined by fine, regularly arranged chromatin granules; karyosome is relatively small and centrally located

A

Entamoeba histolytica

27
Q

Cystic Stage

shape: spherical
size: 5 - 20 miera

A

Entamoeba histolytica

28
Q

Cyst

smooth, definite, relatively thin cystic wall

the number of nuclei range from 1-4, rarely more, with a centrally located
karyosome

in the younger cyst, the chromatoidal bars with rounded ends and glycogen mass may be present

A

Entamoeba histolytica

29
Q

18 H preparation
cytoplasm is grayish violet, granular

the nuclei are clearly seen with dark stained central karyosome

if present, the chromatoidal bars are blue-black in color

if present, the glycogen mass is unstained

A

Entamoeba histolytica

30
Q

NSS preparation

the cyst wall is refractile and relatively thin

the cytoplasm is colorless with a ground glass appearance nuclei are rarely visible, sometimes one may appreciate the outline of the nucleus

if present, the glycogen mass is a clear or empty space in the cytoplasm

if present, the chromatoidal bars are refractile

A

Entamoeba histolytica

31
Q

ladine preparation
.
The cytoplasm is greenish yellow

The nuclei are clearly seen with central karyosome

If present, the chromatoidal bars are refractile

If refractile, the glycogen mass is dark yellowish brown in color

A

Entamoeba histolytica

32
Q

The nucleus has a central karyosorne, RBC are found in the food vacuoles of the trophozoites

the cysts has 1-4 nuclei,

chromatid bars are with rounded ends

A

Entamoeba histolytica

33
Q

The nucleus has a large eccentric karyosome

the cytoplasm of the trophozoite is dirty and highly vacuolated

the cyst has 1 - 8 nuclei

chromatoidal bars are with shard ends.

A

Entamoeba coli

34
Q

The nucleus has a large irregular karyosome which is either centrally eccentrically located

the small cyst has 1 - 4 nuclei.

A

Endolimax nana

35
Q

The nucleus has a large central or eccentric karyosome surrounded by an unstained halo

medium-sized, cyst with characteristic glycogen vacuole seen as an empty space.

A

Iodamoeba buetschlii

36
Q

Usually binucleated

The nucleus is seen with a karyosome consisting of 4-8 chromatin granules

Only the trophozoite stage has been described.

A

Dientamoeba fragilis

37
Q

The trophozoite is binucleated** with **4 pairs of flagella*

a pair of sucking discs

parabasal body

the cyst is football shaped with 1-4 nuclei and crossed fibrils.

A

Giardia lamblia

38
Q

Trophozoite is pear shaped with a single nucleus, cytosome, spiral groove and flagella, cyst is unirrucleated with visible cytosome

A

Chilomastix mesnili

Parasitology LAB (37 decks)

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