AMOEBA

Question 1

Amoebas

Answer 1

Entamoeba coli

Endolimax nana

Iodamoeba buetschlii

Entamoeba gingivialis

Entamoeba histolvtica

Question 2

Trophozoite Stage (I & H preparation)

Shape: condensed, thickened, rounded
mass size: 15 - 50 micra

Answer 2

Entamoeba coli

Question 3

Trophozoite

cytoplasm is grayish violet; there is no clear demarcation between the ectoplasm and endoplasm

the endoplasm is highly vacuolated containing bacteria

the nucleus is spherical with thick nuclear membrane lined with coarse irregular chromatin granules or plaques; the karyosome is moderately large and eccentrically located

Answer 3

Entamoeba coli

Question 4

Cystic Stage

shape: spherical
size: 10 - 33 micra

Answer 4

Entamoeba coli

Question 5

Cyst

smooth, definite, thick cystic wall

number of nuclei range from 1 - 8 rarely more with an eccentric karyosome

in younger cysts, chromatoidal bars with splintered ends and glycogen mass may be present

Answer 5

Entamoeba coli

Question 6

1 & H preparation cytoplasm is granular, grayish violet
nuclei are clearly visible with dark stained eccentric karvosome
• if present, chromatoidal bodies are bluish black
• if present. glycogen mass is unstained

Answer 6

Entamoeba coli

Question 7

NSS preparation
refractile cyst wall cytoplasm is colorless
nuclei are fairly visible as refractile rings
• if present, the chromatoidal bodies are refractile structures
• if present, the glycogen mass is a clear or empty space in the cvioplasm

Answer 7

Entamoeba coli

Question 8

lodine preparation
• cytoplasm is yellowish brewn
• nuclei are clearly seen with eccentric karyosome
• if present, chromatoidal bodies are refractile
• if present, glycogen mass is dark brown in color

Answer 8

Entamoeba coli

Question 9

Trophozoite Stage (I & H preparation)

shape: irregular
• size: small, 6 - 15 micra

Answer 9

Endolimax nana

Question 10

Trophozoite

cytoplasm is grayish-violet, vacuolated which may contain bacteria

there is small, spherical, single nucleus with a large irregular, central or eccentric karyosome

Answer 10

Endolimax nana

Question 11

Cystic Stage

shape: oval
• size: 6 - 10 micra

Answer 11

Endolimax nana

Question 12

Cyst

smooth, definite cystic wall number of

nuclei ranges from 1-4

Answer 12

Endolimax nana

Question 13

• I & H preparation

cytoplasm is grayish, granular

nuclei are visible as darkly stained dots

chromatoidal bodies occasionally seen as deeply stained, small, slightly curved rods

Answer 13

Endolimax nana

Question 14

NSS preparation

refractile cyst wall

cytoplasm is colorless;

nuclei are faintly visible

Answer 14

Endolimax nana

Question 15

lodine preparation

cytoplasm is yellowish

nuclei are visible as dots

glycogen mass is usually absent; if present, it is usually diffused with ill-
defined margins

Answer 15

Endolimax nana

Question 16

Trophozoite Stage (I & H preparation)

shape: irregular
shape: 6 - 25 micra

Answer 16

Iodamoeba buetschlii

Question 17

Trophozoite

granular, vacuolated cytoplasm and vacuoles may contain bacteria and yeast cells

single, spherical nucleus with a large central or eccentric karyosome surrounded by an unstained halo

Answer 17

Iodamoeba buetschlii

Question 18

Cystic Stage
Shape: irregular
size: 6 - 15 micra

Answer 18

Iodamoeba buetschlii

Question 19

Cyst

smooth, definite cystic wall

have a single nucleus, rarely with 2 nuclei

large glycogen vacuole

Answer 19

Iodamoeba buetschlii

Question 20

1& H preparation
• vio et-gray granular cytoplasm

nucleus is visible

large glycogen vacuole is unstained
• chromatoidal bodies are usually absent; if present, they are seen as deeply stained small rods

Answer 20

Iodamoeba buetschlii

Question 21

NSS preparation
• retractile, relatively thick cyst wall cytoplasm is colorless
• nuclei is not visible
glycogen vacuole is unstained or an empty space in the cytoplasm

Answer 21

Iodamoeba buetschlii

Question 22

lodine preparation

cytoplasm is yellowish

nucleus may be visible

glycogen vacuole is clark brown

Answer 22

Iodamoeba buetschlii

Question 23

Trophozoite Stage (I & H preparation)

Shape: irregular
shape: 5 - 35 micra

Answer 23

Entamoeba gingivialis

Question 24

Trophozoite

clear demarcation between the clear ectoplasm and granular endoplasm

endoplasm contains food vacuoles with bacteria and rarely RBC

possesses a single nucleus which is spherical in shape, karyosome is near the center

have multiple pseudopodia with progressive directional movement similar to E. histolytica

Answer 24

Entamoeba gingivialis

Question 25

Trophozoite Stage (I & H preparation) shape: irregular shape: 10--60 micra

Answer 25

Entamoeba histolytica

Question 26

Trophozoite cytoplasm is grayish violet with a clear demarcation between the ectoplasm and endoplasm endoplasm is briefly granular with ingested bc which are bluish black in color single, spherical nucleus; nucleus membrane is lined by fine, regularly arranged chromatin granules; karyosome is relatively small and centrally located

Answer 26

Entamoeba histolytica

Question 27

Cystic Stage shape: spherical size: 5 - 20 miera

Answer 27

Entamoeba histolytica

Question 28

Cyst smooth, definite, relatively thin cystic wall the number of nuclei range from 1-4, rarely more, with a centrally located karyosome in the younger cyst, the chromatoidal bars with rounded ends and glycogen mass may be present

Answer 28

Entamoeba histolytica

Question 29

18 H preparation cytoplasm is grayish violet, granular the nuclei are clearly seen with dark stained central karyosome if present, the chromatoidal bars are blue-black in color if present, the glycogen mass is unstained

Answer 29

Entamoeba histolytica

Question 30

NSS preparation the cyst wall is refractile and relatively thin the cytoplasm is colorless with a ground glass appearance nuclei are rarely visible, sometimes one may appreciate the outline of the nucleus if present, the glycogen mass is a clear or empty space in the cytoplasm if present, the chromatoidal bars are refractile

Answer 30

Entamoeba histolytica

Question 31

ladine preparation . The cytoplasm is greenish yellow The nuclei are clearly seen with central karyosome If present, the chromatoidal bars are refractile If refractile, the glycogen mass is dark yellowish brown in color

Answer 31

Entamoeba histolytica

Question 32

The nucleus has a ***central karyosorne, RBC are found in the food vacuoles*** of the trophozoites the ***cysts has 1-4 nuclei,*** chromatid bars are with ***rounded ends***

Answer 32

Entamoeba histolytica

Question 33

The nucleus has a ***large eccentric karyosome*** the cytoplasm of the trophozoite is ***dirty and highly vacuolated*** the cyst has ***1 - 8 nuclei*** chromatoidal bars are with ***shard ends.***

Answer 33

Entamoeba coli

Question 34

The nucleus has a ***large irregular karyosome*** which is either centrally eccentrically located the small cyst has ***1 - 4 nuclei.***

Answer 34

Endolimax nana

Question 35

The nucleus has a ***large central or eccentric karyosome*** surrounded by an ***unstained halo*** medium-sized, cyst with characteristic glycogen vacuole seen as an empty space.

Answer 35

Iodamoeba buetschlii

Question 36

Usually ***binucleated*** The nucleus is seen with a karyosome consisting of ***4-8 chromatin granules*** ***Only the trophozoite stage*** has been described.

Answer 36

Dientamoeba fragilis

Question 37

The trophozoite is ***binucleated** with ***4 pairs of flagella*** a pair of sucking discs parabasal body the cyst is football shaped with 1-4 nuclei and crossed fibrils.

Answer 37

Giardia lamblia

Question 38

Trophozoite is ***pear shaped*** with a single nucleus, cytosome, ***spiral groove*** and flagella, cyst is unirrucleated with visible cytosome

Answer 38

Chilomastix mesnili