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Parasitology LAB > Microscope > Flashcards

Microscope Flashcards

(75 cards)

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1
Q

One lens
Simplest – magnifying glass or pair of reading glasses

A

Simple microscope

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2
Q

Multiple lens

A

Compound microscope

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3
Q

– the power of the microscope to enlarge the image of an object

A

Magnification

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4
Q

– ability of the microscope to distinguish two objects from each other

A

Resolution

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5
Q

PARTS OF MICROSCOPE
Has 2 major parts:

A

Optical parts – magnifies tissue specimens
Mechanical parts – supports the optical parts

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6
Q

Optical parts

A

Ocular lens
Objective lenses

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7
Q

Magnification of ocular lens

A

10X

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8
Q

Red

A

Scanner

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9
Q

Yellow

A

LPO

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10
Q

Blue

A

HPO

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11
Q

B&W

A

OIO

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12
Q

Holds the different objective lenses
It allows the rotation of the lenses while viewing

A

Revolving Nosepiece

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13
Q

Supports the lenses and nosepiece of the microscope and connects to the base of the microscope

A

Arm

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14
Q

Helps in controlling the amount of light that is passing through the opening of the stage

A

Iris diaphragm

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15
Q

Gathers light from the microscope’s light source and concentrate it into a cone of light that illuminates the specimen

A

Condenser

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16
Q

Lower or raise the stage

A

Coarse adjustment knobs

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17
Q

Used for focus on scanning. Usually, the low power lens is used, enabling the movement of the tube.

A

Coarse adjustment knobs

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18
Q

Used for focusing on high power and oil objectives.

A

Fine adjustment knobs

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19
Q

Moves the body tube for focusing when using the high power lens.

A

Fine adjustment knobs

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20
Q

Provides support to help microscope stand upright

A

Base

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21
Q

Primary magnifier of microscope

A

Objective lens

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22
Q

Maintains the correct distance between the ocular and objective lens

A

Ocular tube

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23
Q

Holds the objective lens.
Its rotation helps to change the power of the objective lens

A

Revolving nose piece

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24
Q

Place for holding sample

A

Stage

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25
Keeps the slide with a specimen in place on the stage
Stage clips
26
Structural or Mechanical Components
Head Arm Base Turret Stage/Stage clips Feedknobs Coarse and Fine Adjustment Knobs
27
Regulates or controls the light (contrast)
Diaphragm
28
So that light can reach the specimen
Aperture
29
Holds the specimen slide
Stage clip
30
Directs the light from the source to the specimen
Condenser
31
Light source
Illuminator
32
Initial focusing
Coarse adjustment knob
33
Fine focusing
Fine adjustment knob
34
Adjust the intensity of light
Rheostat
35
- The distance between the two eyepieces (left and right), usually adjustable to fit individual users.
Interpupillary distance
36
- objectives that can be changed with minimal or no refocusing.
Parfocal
37
A microscope that is “______” is one which, if it is in focus with one objective, when the objective is rotated, will remain (mostly) in focus.
Parfocal
38
If microscope objectives are not_______ it means that you would need to refocus the microscope each time the objective turret is changed to a different magnification.
parfocalled
39
- the object in the center of view will remain in the center when the objective is rotated.
Parcentered
40
Alignment
Parcentered
41
_______ of a microscope is a function of refractive index.
Resolving power
42
The resolution limit of a microscope is the shortest distance between two nearby objects when the images formed by the microscope are properly differentiated.
Resolving power
43
Oil and slides have the same ______
Refractive index
44
- the amount of distance required between the objective lens and the top of the object in order for the sample to appear in focus.
Working Distance
45
Determined by multiplying the power of the eyepiece (ocular) by the power of the objective lens.
Total Magnification Power
46
DO'S and DON'TS (Microscope)
Keep the microscope covered with a clean plastic or cloth cover when not in use. Use a soft cloth dampened with xylene to clean immersion oil from the OlO. Then, polish with a clean lint free cloth. When preparing the microscope for storage, make sure that the objective lens facing the stage is scanner and the stage is brought down Manipulate the light intensity adjustment knob to reduce the light emitted by the illuminator to minimum; then, turn off the light switch and unplug.
47
Keep the microscope covered with a______ or ______ when not in use.
clean plastic or cloth cover
48
Use a soft cloth dampened with______ to clean immersion oil from the OlO. Then, polish with a clean lint free cloth.
xylene
49
When preparing the microscope for storage, make sure that the objective lens facing the stage is_____ and the stage is brought____
scanner down
50
Manipulate the______ to reduce the light emitted by the illuminator to minimum; then, turn off the light switch and unplug.
light intensity adjustment knob
51
DO'S and DON'TS (Slides)
Reject slides that are: imperfectly cleaned surface scratch or notched edge frosted appearance Always handle cleaned slides by the edges. Used slides should initially be placed in water containing a detergent. Then, slides will be cleaned one by one and transferred to a fresh solution of detergent. Rinse then dry with a clean cotton cloth. Slides are best stored in a dry place. It is recommended that cleaned slides be stored by 10s.
52
Reject slides that are:
imperfectly cleaned surface scratch or notched edge frosted appearance
53
Always handle cleaned slides by the_____.
edges
54
Used slides should initially be placed in water containing a______. Then, slides will be cleaned one by one and transferred to a fresh solution of detergent. Rinse then dry with a clean cotton cloth.
detergent
55
Slides are best stored in a
dry place
56
It is recommended that cleaned slides be stored by
10s
57
Stool Specimens Wear:
protective safety glasses gloves laboratory coat
58
Stool specimen Use of____________ when needed.
biological safety cabinets
59
Stool specimen DO NOT:
eat drink smoke apply cosmetics manipulate contact lenses in work area
60
Stool specimen ______work surface and after spill or potentially infectious material.
Decontaminate
61
Stool specimen If you have cuts or abrasions on the skin on your hands, cover them with_____ dressing.
adhesive
62
Stool specimen Dispose "sharps" in sharp container for______.
decontamination
63
Stool specimen Remove_____ and wash____ after completing any task
gloves hands
64
Blood Specimens
Wear PPE If you have cuts or abrasions on the skin on your hands, cover them with adhesive dressing. Use needles and lancets only once and dispose them in "sharp' container for decontamination. Remove gloves and wash hands after completing any task
65
- most commonly used specimen in the diagnosis of parasitic infections
Stool
66
Other specimen used for diagnosis of parasitic infections-
blood sputum urine body fluids tissue biopsy
67
Fecal specimen Collection - need proper instructions and proper containers
type of container size age of specimen substances that may interfere with examinations
68
Condenser Wet mount
Lower the condenser
69
Condenser Stained sample
Condenser near the stage
70
Labelling
Name of patient Age Sex Time of collection What specific type of test
71
Type of container
Scree type Tightly sealed Clean
72
Size of specimen
2-3 tbsp
73
Age if soecime
1hr ideally
74
Substances that may interfere with examination
Antibiotics and many more
75
Stool Fixatives:
10% Formalin LV-PVA SAF Schaudinn's Fixative Modified PVA Cu or Zn One-vial Fixatives

Parasitology LAB (37 decks)

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