Amylase Methodologies Flashcards
Measures the disappearance of starch substrate
a. Amyloclastic
B. Saccharogenic
C. Chromogenic
A. Amyloclastic
Amyloclastic
Starch-Iodine complex (dark blue)→—— in color intensity
Decrease or increase?
Decrease
Amyloclastic
the indicator; only react in polysaccharide
Iodine
can be used as a substitute/alternative substrate and when complexed with iodine produces mahogany brown colored product
A. Glycoside
B. Glycogen
C. Lipase
B. Glycogen
Amyloclastic
The absorbance of the product is —— proportional to the activity of amylase
A. inversely proportional
B.directly
A
Measures the appearance of the product
A. Saccharogenic
B. Chromogenic
C. Amyloclastic
Saccharogenic
reducing sugars
Starch
Saccharogenic
The concentration of the reducing sugar is —— . proportional to the amylase activity
A. Inverse
B. Direct
Direct
Measures the increasing color from production of product- chromogenic dye fragment
A. Amyloclastic
B. Chromogenic
B. Chromogenic
Insoluble starch-dye soluble starch dye fragments
A. Amyloclastic
B. Chromogenic
B. Chromogenic
Chromogenic
Product and amylase activity is—— proportional
A. Directly
B. Inversely
Directly
Coupling of several enzyme systems monitor amylase activity
A. Continuous monitoring
B. Amyloclastic
C. Chromogenic
A
Continuous monitoring is —- proportional
Directly
Increase in absorbance at 340nm is measured
A. Continuous monitoring
B. Amyloclastic
C. Chromogenic
A. Continuous
Continuous monitoring reaction chemical
A.5,6- phosphogluconolactone+ 5 NADH
B. A.5,6- phosphogluconolactone+ 5 NAD
A.5,6- phosphogluconolactone+ 5 NADH
