Animal Models of Depression Flashcards
(23 cards)
which species is easier to genetically manipulate and has been better characteristiced mice or rats ?
mice
do mice or rats preform better in behavioral tests ?
rats
is it better to house animals together or apart ?
together otherwise they become stressed
how is adhedonia modelled ?
sucrose preference test
test if reduced ICSS
test if reduced social inertaction
how is abnormal weight loss modelled?
measure weight loss after stressors
how is insomnia/ excessive sleeping modelled ?
electroencephalography measure abnormal sleep architecture
modelling psychomotor agitation or slowness ?
difficulty handling
locomotor tests
modelling fatigue / loss of energy ?
measure activity in home cage - wheel running
nesting building
active waking (electroencephalography)
modelling indecisiveness / inability to think or concentrate ?
defects in spatial working memory
what aspects of depression cant be modelled ?
recurrent thoughts of dead/ suicide
feelings of worthlessness
most validated tests for depressive like behaviours in rodents ?
Forced Swim Test Tail Suspension Test Olfactory Bulbectomy Learned helplessness DRL-72 (reinforcement behavior)
forced swim test ?
rodents dont like to swim
measure time rodent spends climbing / moving
floating indicates given up - depressive like behaviour
NA deficient mice lack responses to what ?
antidepressentant drugs
what is an olfactory bulbectomy ?
Bilateral removal of olfactory bulbs results in a complex constellation of behavioural and biochemical changes
Many are reversible by chronic but not acute antidepressants
Hyperactivity in an open field is one of the most reliable features
transient inactivation of which cortex leads to antidepressant like affects ?
infralimbic
rodents exposed to CUS have shown to ?
decrease in sucrose intake (Willner et al., 1987)
decrease in reward as measured by ICSS (Moreau et al., 1992
can be reversed by admin of antidepressants
is CUS a reliable model ?
no
many negative reports in the rat
socail defeat stress
put rodent in with more agressive rodent
then put them in again but aggressive rodent is restricted from moving
measure if rodent goes towards aggressor or not
what are wistar kyoto rats ?
very depressed/anxious rat strain
why are WKY rats a good model ?
strong face / predictive validity
what happens to hippo c in WKY rats ?
atrophy
what antidepressant drugs dont WKY rats respond to ?
SSRIs
what deficits do maternally seperated rats have ?
increased inflammatory cytokines
alterations to microbiota
