Antibodies

Question 1

Monoclonal antiBs are

Answer 1

monospecific and identical

Question 2

All therapeutic antiB’s are

Answer 2

IgG

Question 3

Most of the antibodies have a _______ Fc region and a ________ Fab region

Answer 3

human -umab

murine- momab

Question 4

a mixed human/murine antiB is a

Answer 4

chimeric antiB

Question 5

xi means

Answer 5

chimerci

Question 6

zu means

Answer 6

humanized

Question 7

suffix for all therapeutic antiBs is

Answer 7

mab

Question 8

lim is fot

Answer 8

immune system

Question 9

ci is for

Answer 9

cardiovascular

Question 10

tu is for

Answer 10

tumor

Question 11

vi is for

Answer 11

virus

Question 12

Administration varries and are best given

Answer 12

IV

Question 13

AnitBs are disctibuted

Answer 13

extracellularly

Question 14

AntiB can cross BBB, T/F

Answer 14

False, too big

Question 15

Protects IgG from degredation and proloungs the serum 1/2 life

Answer 15

Neonatal Fc REceptor for IgG

Question 16

Helps transfer passive immunity to fetus

Answer 16

Neonatal Fc Receptor for IgG

Question 17

What pH does FcRn bind the Fc regsion of IgG at?

Answer 17

pH lower then 6.5 so releases as 7.4

Question 18

do murine IgG bind human FcRn?

Answer 18

no they don’t

Question 19

Fab antiB fragrements lacking Fc region will

Answer 19

not bind to FcRn

Question 20

This protects the antiB from degredation once it’s taken up into a cell

Answer 20

Neonatual Fc Receptor

Question 21

Half life of IgG1, IgG2, IgG4 is

Answer 21

long or about 21 days

Question 22

IgG3 half life it

Answer 22

7 days

Question 23

antibody fragments Fab have T=1/2 of

Answer 23

.5-21 hours, very short

Question 24

These cells have Fc receptors that can bind and causes endocytosis of IgG

Answer 24

phagocytic cells in target tissue

Question 25

What is the function of renal and biliribin in degredation of antibodies?

Answer 25

unimportant

Question 26

Uses for Monoclonal aniB therapy

Answer 26

``` immunosuppression Autoimmune malignancies infectious diseas asthma ```

Question 27

Direct affect of antiBs on antiG

Answer 27

antagonism or neutralization

Question 28

AntiB will bind and inactivate a

Answer 28

soluble antiG

Question 29

Anitbody acts as competitive inhibitor of ligand by

Answer 29

binding to cellular recpetor

Question 30

Blocking this guy will block neurovasculation

Answer 30

blocking VEGF

Question 31

Blocking VEGF is good for

Answer 31

reducing vascularization of tumors

Question 32

use to tx metastatic colorectal cancer, pancreatic cancer or breast and prostate carcinoma

Answer 32

VEGF blockig by antiB

Question 33

This method of antiB binds and inhitibts EGF receptor to inhibit tumor growth

Answer 33

EGF

Question 34

This antiB targets ______ to stop head and neck or colorectal cancer

Answer 34

EGF

Question 35

By binding and inhibiting the EGF receotpr, the antiB is

Answer 35

inhibiting signaling

Question 36

When would a Chimeric Fab fragment be useful

Answer 36

if we just want a short durationg of action

Question 37

This is good for platele aggregation and inhibits fibrinogen

Answer 37

Chimeric Fab fragmet of mAb

Question 38

therapies for Fab fragrment of mAG

Answer 38

acute coronary syndrome of percutaneous coronary intervention

Question 39

By binding to the Fc Receptor on NK cell, the monoclonal anitB will activated

Answer 39

Anti-body dependent cellular cytotoxicity

Question 40

Rituximab will bind to protein:

Answer 40

CD20

Question 41

whhat is the significance of binding to CD20 on cell surface of B cells

Answer 41

causes B cell lysis when we have leukimic or lymphoma

Question 42

CDC stands for

Answer 42

complement dependent cytotoxicity

Question 43

AntiB bind to cell surface and Fc region binds to

Answer 43

complement protein to activate CDC

Question 44

What is the result of CDC

Answer 44

cell lysis

Question 45

What is a benefit of binding to or inhibiting EGF

Answer 45

we block EGF, get ACDD and CDC and inhibut tumor growth

Question 46

To turn on antibody dependent cellular cytotoxicity (ADCC) we will activate which little guy

Answer 46

C3d

Question 47

Selective delivery of toxins or radio nucleotides work by:

Answer 47

antiB-toxin --> antiB binds to target and is internalized--> toxin released

Question 48

CD30 is a good target for Fab regions to bind to because

Answer 48

CD30 is not in normal cells but is expresed in lyphoma

Question 49

CD30 specific antiB will also bring this along to release in these cells

Answer 49

microuble toin monomtl auristatin E or MMAE t

Question 50

microtublue toxin-monomethy auristatin E is good for

Answer 50

bringing along with CD30 specific IgG because it will stop cell proliferation

Question 51

A CD30 specific drug

Answer 51

Bretuximab vedotin

Question 52

Bretuximab Vedotin works by

Answer 52

Cd3 speficif, has MMAE that stops cell porliferation and then promotes cellylar cytotoxicity and Anitbody dependendt cellular cytotoxicity

Question 53

Ibritumomab tiuxetan Y labled antiB works agains what membrane protein

Answer 53

CD20

Question 54

CD20 is on :

Answer 54

B cells

Question 55

CD20 on B cells is targeted by

Answer 55

Ibritumomab that has a conjgated radioative Y

Question 56

are monoclonal antiB well tolerated

Answer 56

yes.... generally safe

Question 57

What are some side affects of this tx

Answer 57

mechanism specific, if tartes plateltes at risk for bleeding | if VEGF speciic, you can get tumor lysis syndrome or slow wound healing

Question 58

The longer you tx with an immungenticy drug class

Answer 58

you lose the effect the the thearpy

Question 59

Negative affect of antiB that triggers release of a variety of cytockines

Answer 59

cytokine storm