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Flashcards in Antibody Deck (46)
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1

describe humoral immunity

extracellular bacteria
Blymphocyte
transferred by serum antibodies
secreted antibody

2

decribe cell mediated immunity

intracellular microbes
t lymphocyte
activation of macrophage or lysis of infected cell
transferred by lymphocytes

3

difference between IgG expressing B cell and high affinity B cell

one has low affinity and one has high

4

4 outcomes of the activation of B cells

1. b cell turns into antibody secreting cells (IgM)
2. isotype switch to IgG expressing B cell (low affinity)
3. high affinity B cell formed through affinity maturation
4. keep at a quiet state - memory b cell

5

5 classes of antibodies with difference in heavy chain constant region

IgG/E/D/A/M

6

antibody structure parts

antibody binding region with a variable part
constant region (Fc)

7

IgG structure

two identical heavy and light chains
intamolecular disulfide bridges

8

explain the hinge region

rich in proline residues making it flexible and a target for proteolytic digestion
rich in cysteine residues for disulfide bonds

9

IgM and IgE lack hinge region what do they have instead

CH4 domain

10

what does mercaptoethanol reduction do

eliminates disulfide bonds resulting in 2 H chains and L chains

11

papain digestion results in

2 identical Fab and 1 Fc

12

pepsin digestion results in

Fab(2)
and no Fc

13

what is the two gene model

two separate genes encode a single Ig H or L chain
one gene for the V region the other for the C region

14

number of combinations of VJC in
a) gamma chain
b) kapa chain
c) H chain

a) 480
b) 200
c) 5382

15

gene rearrangements

H chain V region genes rearrange first and then the L chain V region genes
rearrangement occurs in an ordered sequence but theyre random events

16

what are the only known site specific dna rearrangements in vertebrates

Ig and TCR genes

17

H chain DNA rearrangements from the germ cell arrangement

1. D gene joins J gene
2. V gene joins DJ
3. C gene joins VDJ then transcription occurs becoming mRNA
C gene determines what class of antibody

18

L chain DNA rearangements

1. LV gene joins J gene
2. C joins LVJ
C gene represent diff type of light chain

19

____ crosses placenta

IgG

20

monomers

IgA,G,D,E

21

pentamer and hexamer

IgM

22

dimer

IgA

23

IgA properties

secretory
binds to polymeric Ig receptor

24

IgG properties

placental transfer
secondary antibody for response to pathogen
binds macrophage

25

IgM properties

primary antibody response

26

IgD properties

mature B cell marker

27

IgE properties

allergy and parasite reactivity
binds mast cells and basophils

28

3categories of antigenic determinants on antibodies

isotypic
allotypic
idiotypic

29

isotype immunogen antibodies

constant region of Ab is the immunogen
often occur in teh Ab from diff species
Ab from the same species will not generate isotype reaction

30

allotype reaction

due to small diff 1-4aa in the constant region of ab from the same species
these differences can arise due to polymorphism
observed in pregnancy and blood transfusion