Flashcards in Antibody Deck (46)
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1
describe humoral immunity
extracellular bacteria
Blymphocyte
transferred by serum antibodies
secreted antibody
2
decribe cell mediated immunity
intracellular microbes
t lymphocyte
activation of macrophage or lysis of infected cell
transferred by lymphocytes
3
difference between IgG expressing B cell and high affinity B cell
one has low affinity and one has high
4
4 outcomes of the activation of B cells
1. b cell turns into antibody secreting cells (IgM)
2. isotype switch to IgG expressing B cell (low affinity)
3. high affinity B cell formed through affinity maturation
4. keep at a quiet state - memory b cell
5
5 classes of antibodies with difference in heavy chain constant region
IgG/E/D/A/M
6
antibody structure parts
antibody binding region with a variable part
constant region (Fc)
7
IgG structure
two identical heavy and light chains
intamolecular disulfide bridges
8
explain the hinge region
rich in proline residues making it flexible and a target for proteolytic digestion
rich in cysteine residues for disulfide bonds
9
IgM and IgE lack hinge region what do they have instead
CH4 domain
10
what does mercaptoethanol reduction do
eliminates disulfide bonds resulting in 2 H chains and L chains
11
papain digestion results in
2 identical Fab and 1 Fc
12
pepsin digestion results in
Fab(2)
and no Fc
13
what is the two gene model
two separate genes encode a single Ig H or L chain
one gene for the V region the other for the C region
14
number of combinations of VJC in
a) gamma chain
b) kapa chain
c) H chain
a) 480
b) 200
c) 5382
15
gene rearrangements
H chain V region genes rearrange first and then the L chain V region genes
rearrangement occurs in an ordered sequence but theyre random events
16
what are the only known site specific dna rearrangements in vertebrates
Ig and TCR genes
17
H chain DNA rearrangements from the germ cell arrangement
1. D gene joins J gene
2. V gene joins DJ
3. C gene joins VDJ then transcription occurs becoming mRNA
C gene determines what class of antibody
18
L chain DNA rearangements
1. LV gene joins J gene
2. C joins LVJ
C gene represent diff type of light chain
19
____ crosses placenta
IgG
20
monomers
IgA,G,D,E
21
pentamer and hexamer
IgM
22
dimer
IgA
23
IgA properties
secretory
binds to polymeric Ig receptor
24
IgG properties
placental transfer
secondary antibody for response to pathogen
binds macrophage
25
IgM properties
primary antibody response
26
IgD properties
mature B cell marker
27
IgE properties
allergy and parasite reactivity
binds mast cells and basophils
28
3categories of antigenic determinants on antibodies
isotypic
allotypic
idiotypic
29
isotype immunogen antibodies
constant region of Ab is the immunogen
often occur in teh Ab from diff species
Ab from the same species will not generate isotype reaction
30