Applications/Concepts for Enzyme Kinetics

Question 1

Where are enzymes and substrates present in small amounts?

Answer 1

the cytosol

Question 2

Why are enzyme-substrate interaction non-covalent?

Answer 2

we don’t want permanant bonds because we want the enzyme to keep being reused

Question 3

What do enzymes usually undergo to help stabalize the interaction?

Answer 3

conformational change

Question 4

What 2 things often help alter the active site and help in catalysis?

Answer 4

1. coenzymes(vitamins)
2. cofactors (metal ions)

Question 5

What are the 3 steps in an enzymatic reaction?

Answer 5

1. enzyme locates and binds to correct substrate
2. substrate undergoes reaction to form the product
3. products are released from the enzyme