ATP synthase Flashcards
is e- transport required for ATP synthesis
no, the proton gradient is suffiicient
which part of ATP synthase is on the matrix side
F1
which side of the atp synthase is in the membrne
F0
how does H+ cause rotation
high IMS H+ causes it to move through half unit, binds to acidic residues (aspartic acid) on C ring, causes conformation change and rotation of c ring
rate limiting step
ATP displacement
how many protons needed for each ATP synthesized
3 protons
ETC and ATP are
coupled
uncouplers are a type of
ionophore
uncouplers can ______ ____ across the ____ and carry ___
diffuse freely, H+
DNP allows the _____ to proceeed when _____ is not being ______
ETC, ATP, synthesized
oligomycin blocks ____________ by binding to the _____ channel of _______
ATP synthesis, proton, F0
primary regulator of xoidative phos
ADP availbility
how does h2po4- enter the matrix and what does it cost
proton coupled symporter, one H+
total protons for ATP breakdown
3 (atp synthase) + 1 (Pi transport)
DHAP/G3P shuttle step 1
DHAP reduced by NADH in the cytosol
DHAP/G3P shuttle step 2
G3P reoxidized, fad reduced
DHAP/G3P shuttle step 3
FADH2 sends e- to coq
DHAP/G3P shuttle step 4
DHAP returns to cytsol
energy implications of DHAP/G3P
only got an FADH2 in exchange for a cytoplasmic NADH, take a hit on ATP generation due to immediate need rather than optimal efficiency (mucle, brain)
what causes NADH to be produced in the matrix
reoxidation o fmalate to oxaloacetate
in the malate/aspartate shuttle, what happens first
oxoloacetate is reduced to malate
in the malate/aspartate shuttle, what happens second
malate is transported into the mitochondrial matrix by the malata=aketoglutarate transporter
what happens third in the malata-aspartate shuttle
malate is oxidized into oxaloactetate
nad is reduced to nadh + h
what happens 4th in the malate-aspartate
oxoloaceteate -> aspartate
glutamate -> alpha ketoglutarate
