Bacte Lec: Campylobacter

Question 1

Morphology of campylobacter

Answer 1

S-shaped, spirally curved (one or more spirals), Gram-negative, pleomorphic rods

Question 2

Is Campylobacter motile?

Answer 2

Motile by a single polar flagellum at one or both ends

Question 3

Is Campylobacter oxidase positive?

Answer 3

Yes

Question 4

Does campylobacter use carbohydrates?

Answer 4

Does not use carbohydrates

Question 5

How many species of campylobacter recognized?

Answer 5

18 species

Question 6

Campylobacter species that is pathogenic for animals.

Answer 6

Campylobacter fetus (2 subspecies) and C. jejuni

Question 7

2 subspecies of C.fetus

Answer 7

Subsp. venerealis & Subsp. fetus

Question 8

Cause of bovine genital campylobacteriosis

Answer 8

C. fetus subsp. venerealis

Question 9

C. fetus subsp. venerealis is found in what body part of cattle?

Answer 9

Found in preputial cavity of asymptomatic bull and genital tract of cow and heifer

Question 10

C. fetus subsp. venerealis mode of infection?

Answer 10

Venereal: Present in semen of infected bull

Question 11

Campylobacter that occurs in intestines of cattle and sheep and in the genital tract of infected sheep and cattle

Answer 11

C. fetus subsp. fetus

Question 12

Mode of infection of C. fetus subsp. fetus

Answer 12

Mode of infection is by ingestion, directly or via fomites.

Question 13

After ingestion of C. fetus subsp. fetus what happens?

Answer 13

After ingestion, there is hematogenous spread with infection of the uterine mucosa

Question 14

C. fetus subsp. fetus in fetus, causes what characteristic?

Answer 14

Necrotic foci in fetal liver characteristic

Question 15

What are the secretions in Campylobacter used for culture?

Answer 15

Cervical mucus and preputial secretions for culture

Question 16

T/F: Campylobacter can pass thru 0.25 um membrane filter

Answer 16

False: Pass thru 0.65 um membrane filter

Question 17

T/F: Fatty Acid Typing (FAT) is used to identify C. fetus in preputial washings, cervical mucus and fetal stomach contents

Answer 17

True

Question 18

What type of diagnosis test is used to detect subsp venerealis in semen

Answer 18

PCR

Question 19

T/F: C. jejuni is resistant to HCL

Answer 19

False: C. jejuni killed by HCl

Question 20

T/F: Neutrophils and macrophages in feces suggest they play a role in elimination of the organism

Answer 20

True

Question 21

It is the S-layer protein is immunodominant antigen in C. fetus

Answer 21

99-kDa protein

Question 22

What is the temperature and condition of Blood agar containing antibiotics.

Answer 22

Plates incubated at 37’C in Atmosphere of 10% CO2, 5% O2 and 85% N2

Question 23

In blood agar, what colonies are observed?

Answer 23

Fine, pinpoint colonies after 3 to 6 days incubation

Question 24

Id of Campylobacter spp. isolates based on ___ ribosomal RNA gene sequences

Answer 24

16s rRNA

Question 25

What is used for short duration immunity?

Answer 25

Bacterin composed of C. fetus subsp venerealis with adjuvants such as oil, alum or other compounds

Question 26

Prevention: Semen for AI is treated with what antibiotics?

Answer 26

Streptomycin and penicillin

Question 27

T/F: Using AI only results in eventual elimination of C. fetus subsp. fetus

Answer 27

False: Using AI only results in eventual elimination of C. fetus subsp. venerealis

Question 28

T/F: C. fetus subsp. venerealis can be eliminated from herd but not subsp. fetus

Answer 28

True

Question 29

What C. fetus causes human infection?

Answer 29

C. fetus subsp fetus

Question 30

Campylobacter that occurs as commensal of intestinal tracts of domestic and wild animals including birds, poultry, dogs and cats

Answer 30

Campylobacter jejuni

Question 31

What virulence factor is responsible for the organism's invasiveness and allows colonization?

Answer 31

flagella and adhesin

Question 32

T/F: C. jejuni has the capability to survive in phagosome?

Answer 32

True

Question 33

This virulence factor activates adenylate cyclase which increases levels of cyclic AMP.

Answer 33

Enterotoxin

Question 34

What happens when Adenylate cyclase is activated?

Answer 34

Increases levels of cyclic AMP resulting to fluid loss into the lumen of the intestine, thereby, diarrhea

Question 35

Virulence factor of C. jejuni that causes local tissue destruction and abscess formation.

Answer 35

Cytotoxin

Question 36

C. jejuni causes infections characterized by?

Answer 36

diarrhea and enterocolitis

Question 37

C. jejuni causes what in chickens and turkeys?

Answer 37

Avian infectious hepatitis

Question 38

What antibiotic is mixed with drinking water that is an effective treatment of C. jejuni

Answer 38

erythromycin