Bacte Lec: Pseudomonas

Question 1

Pseudomonas is usually found in?

Answer 1

Commonly found in aquatic habitats and soil

Question 2

P. that is specialized mammalian parasite.

Answer 2

P. mallei

Question 3

Are pseudomonas motile?

Answer 3

All motile by means of polar fl agella except P. mallei

Question 4

Common description of pseudomonas

Answer 4

Rod-shaped and oxidase positive

Question 5

Other name for P. aeruginosa

Answer 5

Bacillus of green pus, P. pyocyaneus

Question 6

Low virulence but cause suppurative infections in domestic animals

Answer 6

P. aeruginosa

Question 7

Common in intestinal contents of chickens and can result in early spoilage of meat

Answer 7

P. aeruginosa

Question 8

Causes “green wool” in sheep

Answer 8

P. aeruginosa

Question 9

P. aeruginosa morphology

Answer 9

Straight, slender rod about 2.5 x 0.4 um. Gram negative. Motile by one to three polar flagella

Question 10

Does P. aeruginosa form spores?

Answer 10

Spores not formed and capsule sometimes present

Question 11

P.aeruginosa produce what pigment in colonies?

Answer 11

Recognized by bright green pigment and grape-like odor (aminoacetophenone) it produces

Question 12

Pigment of P.aeruginosa is consists of what?

Answer 12

fluorescein, a pyoverdine which is yellow-green and becomes oxidized to yellow and pyocyanin, a bluish green pigment that oxidizes to brown

Question 13

T/F: P.aeruginosa can be cultivated in simplest media

Answer 13

T: Can be cultivated in simplest media

Question 14

Produce smooth, shiny, moist, fimbriate and spreading colonies

Answer 14

P.aeruginosa colonies

Question 15

P.aeruginosa incubation temperatures

Answer 15

Grows on wide range of incubation temperatures, 4 to 42’C

Question 16

P.aeruginosa is positive in what?

Answer 16

Does not ferment carbohydtares but some strains produce acid from some sugars

catalase and oxidase positive

Question 17

P.aeruginosa is negative in what?

Answer 17

Indole negative, MR negative, VP

Question 18

Does P.aeruginosa liquefy gelatin?

Answer 18

Most strains from lesions liquefy gelatin

Question 19

Antigens of P.aeruginosa

Answer 19

17 somatic O antigens, 6 H or fl agellar antigens

Question 20

What toxin is found in P.aeruginosa

Answer 20

toxin A, alkaline proteases and elastase which destroys elastin in lung parenchyma

Question 21

T/F: Toxin A helps in protein synthesis.

Answer 21

F: Toxin A inhibits protein synthesis and contributes signifi cantly to lesion development in burn infections

Question 22

Protein known as ____ which is used in typing by testing extract of an unknown organism against indicator strains

Answer 22

pyocin

Question 23

They are component of the normal bacterial flora of the skin, mucous membranes and intestines of healthy animals

Answer 23

P.aeruginosa

Question 24

____ and ____ important in production of edema, induration, hemorrhage and necrosis observed in skin lesions

Answer 24

Toxin A and protease

Question 25

_____ produced by P. areuginosa organism antiphagocytic and facilitates organisms’ penetration of tissues

Answer 25

Slime layer

Question 26

P. aeruginosa is found in what diseases?

Answer 26

necrotic pneumonias, enteritis and rhinitis in swine, lesions associated with traumatic reticulopericarditis in cattle

Question 27

causes sporadic abortion in cattle, spermicidal and causes balanoposthitis in bulls

Answer 27

P. aeruginosa

Question 28

P. aeruginosa causes what in dogs?

Answer 28

Causes suppurative otitis externa in dogs as complications of injury, mange, bacterial and fungal infections