BACTE MTAP Flashcards

1
Q

Obtain during acute phase of infection within how many days for viruses?

A

2-3 days

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2
Q

Deliver to lab within how many minutes of collection?

A

30 minutes

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3
Q

Specimens should be transported to the laboratory within how many hours of collection

A

2 hours

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4
Q

Microorganism that is susceptible to environmental conditions such as TEMPERATURE?

A

Neisseria meningitidis

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5
Q

Microorganism that is susceptible to environmental conditions such as pH?

A

Shigella

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6
Q

Specimens for VIRUSES, CHLAMYDIA, and MYCOPLASMA are usually submitted in appropriate transport media at what temperature to stabilize respective microorganisms?

A

4 degrees celsius

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7
Q

What is the transport medium for bacterial and viruses?

A

STUART MEDIUM

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8
Q

Risk group that AGENTS NOT ASSOCIATED with disease in healthy adult humans.

A

Risk group 1

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8
Q

Risk group that agents associated with human disease that is RARELY SERIOUS and for which preventive or therapeutic interventions are often available.

A

Risk Group 2

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9
Q

Agents associated with SERIOUS OR LETHAL human disease for which preventive or therapeutic interventions MAY BE AVAILABLE

A

RISK GROUP 3

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10
Q

Agents likely to cause serious or lethal human disease for which preventive or therapeutic interventions are NOT USUALLY AVAILABLE

A

RISK GROUP 4

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11
Q

Exhaust system that removes particles equal to, or greater than 0.3 microns, which essentially include all bacteria, spore and viruses, with an efficiency of 99.97%. HEPA filters are effective at trapping particulates.

A

HEPA (High Efficiency Particulate Air) filter

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12
Q

A hood with high-efficiency particulate air (HEPA) filters that provides personnel, environmental and/or product protection when appropriate practices and procedures are followed.

A

BSC (Biological Safety Cabinet) or biosafety cabinet

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13
Q

IMPROPER SWAB (CALCIUM ALGINATE)

A

VIRUSES

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14
Q
A
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15
Q

IMPROPER SWAB (WOOD)

A

CHLAMYDIA

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16
Q

WHAT ARE THE GROWTH INHIBITOR OF BACTERIA?

A

FATTY ACIDS

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16
Q

IMPROPER SWAB (COTTON SWAB)

A

NEISSERIA, BARTONELLA , SOME ANAEROBIC BACTERIA

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17
Q

Basic Media

A
  • Nutrient Broth
  • Nutrient Agar
  • Saboraud’s Broth
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18
Q

Enriched Media (Solid)

A
  • Blood Agar
  • Chocolate Agar
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19
Q

Selective Media

A

MacConkey’s Agar

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20
Q

Enrichment Media (Liquid)

A

Selenite Broth

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21
Q

Differential Media

A

Blood Agar

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22
Q

Medium for Anaerobic spp.

A

Thiogelycollate

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23
Media for Mycobacteria
The Lowenstein-Jensen medium Appearance: Brown, granular colonies "buff", rough and tough
24
Media for FUNGI
- Sabouraud dextrose - Malt extract - Brain heart infusion medium
24
What is added to prevent contamination of the medium by bacteria
Chloramphenicol
25
Examples of CHROMOGENIC MEDIA
- chromAgar - Mannitol Agar
26
Quantitative ( INOCULATION OF MEDIA )
URINE CULTURES
26
SEMI-QUANTITAIVE (INOCULATION OF MEDIA )
PUS CULTURES
27
INCUBATION TEMP. FOR FUNGI
28◦C to 30◦C
28
INCUBATION TIME FOR BACTERIA, VIRUSES AND ACID FAST BACILLUS
35◦C to 37◦C
29
Anaerobe jars, bags, or chambers is composed of?
-5% to 10% hydrogen (H2), -5% to 10% CO2 -80-90% nitrogen (N2), -0% O2.
30
Procedure differentiates organisms of the domain bacteria according to CELL WALL structure.
GRAM STAIN
31
INVENTOR OF GRAM STAIN
HANS CHRISTIAN GRAM
32
MORDANR FOR KINYOUN STAIN
TERGITOL
33
A quick, inexpensive fungal test to differentiate dermatophytes and Candida albicans
Potassium hydroxide preparation or KOH prep
34
Presence of fungal elements in KOH w/ Calcofluor white
Chalk-white or brilliant apple green color
35
A dye that intercalates or binds with the nucleic acid
ACRIDINE ORANGE
36
Selective dye that emits green fluorescence when bound to?
dsDNA at 520nm
36
Emits orange light (ACRIDINE ORANGE)
-lysosomes -bacteria -yeast
36
Red fluorescence when bound to?
RNA at 650nm
37
Appears orange or red with yellow-green nucleus
Trichomonas vaginalis
38
Appears pale green to yellow
Human epithelial and inflammatory cells and tissue debris
39
Ureaplasma and Mycoplasma can be visualized on acridine orange staining but can be differentiated through?
Urease test
40
White pinhead colony appearance
S. areus
41
Bacterias which has this enzyme has the ability to cause anemia
Hemolysin
42
Growth on Loeffler’s Serum Slant (S. areus)
golden yellow pigment
43
Growth on Loeffler’s Serum Slant (S. citreus)
lemon yellow
44
Growth on Loeffler’s Serum Slant (S. albus)
procelain white pigment
45
Inhibitor of MSA
7.5% NaCl
46
PINK COLONIES ON MSA
S. epidermidis and S. saprophyticus
47
YELLOW HALO FORMATION ON MSA
S. areus
48
Between STAPH AND MICROCOCCUS WHO IS SUSCEPTIBLE TO LYSOSTAPHIN AND FURAZOLIDONE BUT RESISTANT TO BACITRACIN?
Staphylococcus
49
Color indicator of Modified oxidase Test
Bromthynol blue
50
Most definitive test to S.areus?
Coagulase Test
51
Enzyme that can possibly dissolve clot
Fibrinolysin / staphylokinase
52
Cell wall, anti-phagocytic, virulence of S.aureu
PROTEIN A
53
#1 wound infection; #1 osteomyelitis (septic arthritis)
S. areus
54
DNASE COLOR (POSITIVE REACTION)
- Toluidine blue ( pink ) - Methyl green ( clear )
55
- Causes endocarditis (associated with Prosthetic Heart Valve Surgery infection) - Novobiocin SUSCEPTIBLE - Non-hemolytic - Blood culture contaminant
S. epidermidis
56
-Causes UTI in young women; Specimen for diagnosis: urine - Novobiocin RESISTANT (less than 16mm)
S. saprophyticus
57
Destroys stratum granulosum of skin
Exfoliatin
58
#1 cause of food poisoning in the Philippines
S. aureus
59
White pinpoint colonies
Streptococcus
60
Selective medium for Streptococcus
Phenyl Ethyl Alcohol Agar
61
NON-ENTEROCOCCUS
- S. bovis ( common cause of Colon cancer) - S. equinis
62
Lancet or dome shape colonies
S. pneumoniae
63
- Leading flesh-eating bacteria (necrotizing fasciitis) - RHD (Rheumatic Heart Disease) / RF (Rheumatic Fever) - AGN (Acute Glumerulonephritis)
S. pyogenes
64
- #1 neonatal meningitis - resistant to all ( Taxo A, P and SXT)
S. agalactiae
65
Differentiates Group D from other Streptococcus
BEA (Bile Esculin Agar)
66
Indicator of BEA
Ferric Ammonium Citrate
67
Color indicator of PYR test
P-dimethylaminocinnamaldehyde
68
Indicator of Sodium Hippurate
Ninhydrin
69
Best test to identify S. pyogenes
Bacitracin Susceptibility Test
70
- rust-colored sputum - #1 adult bacterial meningitis - #1 otitis media
S. pneumoniae
71
VIRIDANS
MUSCISM - mutans - uberis - salivarus - constellatus - intermedius - sanguis - mitis
72
Used to identify S. pneumoniae
Optochin Disk Test (Taxo P) COMPOSITION: ethylhydroxycuprein HCl
73
Detects pneumococcal antibody; skin test for S. pneumoniae infection
Francis Test
74
Detects erythrogenic antigen; skin test for S. pyogenes infection
Dick's test
75
A skin test and immunity test for scarlet fever that uses antitoxin to the erythrogenic toxin of S. pyogenes subcutaneously
Schultz Charlton test
76
Transport system for Neisseria
JEMBEC system
77
Presumptive (screening) test for gram negative cocci
Oxidase Test / Taxo N
78
3rd cause of otitis media (middle ear infection)
Moraxella catarrhalis