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ENTEROBACTERIACEAE Flashcards

1
Q

Most species are normal flora of GI Tract such
E. coli except

A

YSS - Yesernia, Salmonella, Shigella

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2
Q

Enterobacteriaceae are

A

Non – sporulating, facultative anaerobes (able
to survive with or without O2)

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3
Q

All are catalase (+) except

A

Shigella dysenteriae

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4
Q

Most are Cytochrome C oxidase negative
which separates enteric from oxidase positive
bacteria of genera

A

▪ Pseudomonas
▪ Aeromonas
▪ Vibrio
▪ Alcaligenes
▪ Achromobacter
▪ Flavibacterium
▪ Cardiobacterium

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5
Q

O antigen

A

Outer Membrane

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6
Q

H antigen

A

Flagella

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7
Q

K antigen

A

Capsule

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8
Q

Vi antigen

A

Capsule of Salmonella

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9
Q

German word meaning nonspreading

A

Ohne hauch

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10
Q

Among the E. coli, certain O antigens are
associated with specific virulent phenotype:

A

▪E. coli O 111 and E. coli O 125 are found
to cause infantile diarrhea

▪ E. coli O112 is often an invasive strain
that causes dysentery – like syndrome

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11
Q

HEAT STABLE and associated with lipopolysaccharide or endotoxin moiety of the cell wall

A

O – somatic antigen

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12
Q

From German word “Kapsel”

A

K – envelope or capsular antigen

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13
Q

A polysaccharide and Heat Stable

A

K – envelope or capsular antigen

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14
Q

K – envelope or capsular antigen is produced by?

A

KES - Klebsiella, E. coli, Salmonella

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15
Q

From German word meaning spreading

A

Hauch

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16
Q

Heat Labile & Protein in nature

A

H – Flagellar antigen

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17
Q

Enterobacteriaceae are further classified based on their

A
  1. Habitat
  2. Tribes
  3. Lactose fermentation
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18
Q

Coliforms in the Normal Flora – found In GI
TRACT

A

CHEEKS - Citrobacter, Hafnia, E. coli, Enterobacter, Klebsiella, Serratia

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19
Q

Non coliform in Normal Flora – Non-gramnegative bacilli found in GI Tract

A

MEPP - Morganella, Edwardsiella, Proteus, Providencia

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20
Q

True Enteric Pathogens – can cause disease in
GIT

A

YSS - Yersinia , Salmonella, Shigella

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21
Q

True Non – Enteric Pathogens

A

Yersinia Pestis - causes Bubonic
Plague

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22
Q

LACTOSE FERMENTERS, Appears pink (+) in MAC

A

CEEK - Citrobacter, Escherichia, Enterobacter, Klebsiella

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23
Q

NON – LACTOSE FERMENTERS

A

ShYPS - Shigella, Yersinia, Proteus, Salmonella

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24
Q

NON – LACTOSE FERMENTERS are further based on?

A

MOTILITY and H2S Production

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25
Nonmotile, Non – H2s Producers
Shigella, Yersinia
26
Motile, H2s Producers
Proteus, Salmonella
27
is the degradation product of amino acid Tryptophan together with pyruvic acid and ammonia
Indole test
28
Indole is the degradation product of amino acid Tryptophan together with pyruvic acid and ammonia due to enzyme?
Tryptophanase
29
KOVAC’S reagent contain
PDAB - Paradimethylaminobenzaldehyde
30
Detects rapid indole producing organism
Spot Indole Test
31
Requiring overnight incubation which identifies weak indole producing organisms
Conventional Tube Method
32
Indole (+) organisms are
PEMPCK - P. vulgaris, E.coli, M. morganii, Providencia, C. koseri, K. oxytoca,
33
3 COMMONLY USED AGAR MEDIUM
1. SULFIDE – INDOLE – MOTILITY (SIM MEDIUM) 2. MOTILITY – INDOLE – UREASE (MIU) MEDIUM 3. MOTILITY – INDOLE – ORNITHINE (MIO) MEDIUM
34
Methyl Red (+) organisms are:
1. Echerichia coli 2. Shigella species 3. Salmonella species 4. Citrobacter species 5. Proteus species 6. Yersinia species
35
Methyl Red (-) organisms are:
KESH - 1. Klebsiella pneumoniae 2. Enterobacter species 3. Serratia marcescens 4. Hafnia species
36
added as a catalyst or color intensifier to VP test
Alpha – naphthol
37
acetoin is oxidized to
diacetyl
38
Test should not be read after standing for over 1 hour because (-) VP cultures may produce a?
copper like color, potentially resulting in false (+) interpretation.
39
VP POSITIVE ORGANISMS
KESH 1. Klebsiella species 2. Enterobacter species 3. Serratia species 4. Hafnia species
40
sole carbon source
sodium citrate
41
sole nitrogen source.
ammonium salts
42
Product of indole test
Indole + Pyruvic acid + ammonia
43
pH indicator of sodium citrate
bromthymol blue
44
is an alternative test medium, which incorporates phenol red
Christensen’s medium
45
The alkaline carbonates and bicarbonates produced as by products of citrate catabolism raise the pH of the medium to?
to above 7.6, causing the bromothymol blue to change from the original green color to blue
46
Under alkaline conditions, pyruvate is metabolized to
acetate and formate.
47
At pH 7.0 and below, produced what?
lactate and acetoin
48
CITRATE NEGATIVE ORGANISMS
MESSSY (triple s) Morganella, E. coli, Shigella, Salmonella typhi, Salmonella paratyphi A, Yersinia
49
CITRATE VARIABLE ORGANISMS
PVV - P. vulgaris, Vibrio cholerae, V. parahaemolyticus
50
Added to detect the production of hydrogen sulfide gas
Ferrous sulfate and Sodium thiosulfate
51
indicator of H2S formation
Iron (ferrous sulfate)
52
Acts as a source of nitrogen on TSI
peptone
53
A/A, Produces gas
EKE- E. coli, Klebsiella, Enterobacter
54
K/A, Produces gas, H2S positive
SPACE - Salmonella, Proteus, Arizona, Citrobacter, Edwardsiella
55
K/A
Shigella
56
K/K
Pseudomonas
57
can be determined by microscopic examination of bacteria or by observing growth in a semi solid medium
Motility
58
typically give diffuse, hazy growths that spread throughout the medium rendering it slightly opaque
Motile bacteria
59
Delayed positive organisms (E.g., Enterobacter) will no produce a positive reaction due to
high buffering capacity of this medium
60
Used for the presumptive evidence of the presence of Helicobacter pylori in tissue biopsy material.
DIAGNOSTIC USE OF UREASE TEST
61
A positive urease test is considered presence of
Helicobacter pylori
62
UREASE POSITIVE
PUNCH : Proteus, Ureaplasma Nocardia, Cryptococcus, Helicobacter pylori
63
OXIDASE POSITIVE
PVNCH: Pseudomonas, Vibrio, Neisseria, Campylobacter, Helicobacter

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