HISTOPATH MTAP Flashcards

1
Q

Branch of anatomical pathology that deals on the study of diseases in a given tissue or organ

A

HISTOPATHOLOGY

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2
Q

Includes the preparation, processing and staining of tissue sections for microscopic study to be interpreted by the pathologist

A

HISTOTECHNIQUES

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3
Q

Can cause death

A

MALIGNANT

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4
Q

CANNOT CAUSE DEATH

A

BENIGN

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5
Q

FUNDAMENTALS ATHOLOGY

A

F - Forensic
I - Immunohisto
S - Surgical
C - Cytology
A - Autopsy
M - Molecular Biopsy

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6
Q

COMPANY FOR RECORD KEEPING AND SPECIMEN RETENTION

A

COLLEGE OF AMERICAN PATHOLOGISTS

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7
Q

o Specimen

A

1 month – 1 year

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8
Q

o Tissue blocks

A

3 years – 10 years

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9
Q

o Slides

A

Indefinite

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10
Q

o Records

A

Permanent

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11
Q
  • Preparation for organisms
A

WHOLE MOUNT

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11
Q

Result forms

A

TRIPLICATE

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12
Q

WHOLE MOUNT THICKNESS

A

0.2-0.5 mm thickness

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13
Q
  • Majority of preparation
A

SECTIONING

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14
Q

SECTIONING THICKNESS

A

3-5 mm

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15
Q

Derived from blood, bone marrow and bodily fluids

16
Q

For cytological examination

17
Q

Microscopic examination of cells from different BODY SITES for diagnostic purposes

A

DIAGNOSTIC CYTOLOGY

18
Q

Microscopic study of cells that have been DESQUAMATED from epithelial surfaces

A

EXFOLIATIVE CYTOLOGY

19
Q

DERIVATIVES OF DIAGNOSTIC

A

Exfoliative Cytology and Fine Needle Aspiration

20
Q

DERIVATIVES OF EXFOLIATIVE

A

Epithelial & Mucous membrane

21
Q

Observation of physiological processes

A

FRESH TISSUE EXAMINATIONS

22
Q

Also referred to as “Dissection”/”Separation”

A

. TEASING/DISSOCIATION

23
Q

Examine as stained preparation

A

Bright field/Light Microscope

24
Unstained preparation
Phase-Contrast Microscope
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* Used to locate the presence & position of mineral elements in the tissue
MICROINCINERATION
26
* A polarizing microscope is often used to examine the ash residue of the incinerated section
SPODOGRAM
27
* It determines the relationship and location of the isotopes and cells to be studied
AUTORADIOGRAPHY
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Is the process in adopting to kill, harden and preserve tissue materials (from destruction and post-mortem changes
FIXATION
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FACTORS AFFECTING FIXATION/PRESERVATION
1. Size and nature/type of tissue 2. Volume of tissue: 1:20x volume of tissue 3. pH: 6-8 4. Temperature: Room Temperature (20-22OC) 5. Osmolality: slightly hypertonic solution
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PHYSIOLOGICAL PROCESS
-MITOSIS -PHAGOCYTOSIS -PINOCYTOSIS
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FORMALDEHYDE (FIXATES WHAT?)
FMG FATS, MUCIN, GLYCOGEN
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Most widely used for Electron Microscopy
GLUTARALDEHYDE
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LEAD FIXATIVE ( FIXATES WHAT)
ACID MUCOPOLYSACCHARIDE
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NEWCOMER’S FLUID ( FIXATES WHAT?)
mucopolysaccharide; nuclear proteins
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ZENKER’S FLUID ( FIXATES WHAT?)
CLSN -connective tissue, liver, spleen, nuclei
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CARNOY’S FLUID ( FIXATES WHAT?)
CLUB - chromosomes, lymph glands, urgent biopsies - MOST RAPID
37