Bacteriology

Question 1

Gram +ve 
Indole -
MR + 
VP -
Lactose fermenter
Nitrate reducing
Abundant H2S
TSI - all sugars fermented, acid , gas

Answer 1

C. Perfringens (clostridia)
Brick shaped
Double zone hemolysis
Naglers reaction
Myonecrosis
Gas gangrene
Reverse CAMP test

Question 2

Only peculiar bacterial microorganism which is MR and VP positive

Answer 2

S. Aureus

Gram positive cocci in clusters

Question 3

Superantigen of s. Aureus unrelatively also causes

Answer 3

Kawasaki disease

Question 4

Toxins produced by s. Pneumonae

Answer 4

Capsular polysaccharide
Pneumolysin
Pneumococcal autolysin
Hemolysin
Leucocidin

Question 5

Gram -ve
Fast lactose fermentor
Mucoid forming
Non - motile rods
MR -
VP+
Indole -ve
Citrate +
Urease +
Red current jelly sputum

Answer 5

Kleb. Pneumoniae/freed landon bacillus

PPUS

Question 6

Granules of c. Diphtheria which gives it a characteristical beaded appearance are known as

Answer 6

Polymetaphosphate granules/volutin/Babes Ernst granules/metachronatic granules/polar bodies

Question 7

Leishman stain in c . Diptheria used to rule out

Answer 7

Vincent spirochete and fusiform bacilli ( cause vincents angina)

Question 8

Gram +
Pleomorphic
Non-lactose and mannitol fermentor
Club shaped 
Metachromatic granules
Grey colony on blood agar
Acid without gas
Grey/black on tellurite agar
Eleks gel ppt test

Answer 8

C. Diphtheria/ klebs-loefflers bacilli

Question 9

Gram negative
Kidney shaped diplococci
Non enveloped

Answer 9

N. Gonorrhea
Pili
IgA protease
Outer membrane protein

Question 10

Morphological types of pili based on presence of pili (virulence,adhesion,prevent phagocytosis)

Answer 10

T1-T4
1(P+),2(P++)- brown,piliated,virulent
(P-)3,4- granular,non piliated,avirulent

Question 11

Outermembrane protein (lipopolysachharide) of n. Gonorrhoea

Answer 11

Protein 1 and 3 ligands form transmembrane porins
Protein 2 opacity associated (OPA)

Others are IgA protease

Question 12

Fitz-hugh-curtis syndrome is seen in

Answer 12

N. Gonorhhea

Peritonitis,ectopic pregnancy,perihepatic inflammation

Question 13

Pelvic inflammatory disease is commonly seen in….infection

Answer 13

Gonorrhea

Question 14

Clinicals of gonnorhhea

Answer 14

Septic arthritis
Vulvovaginitis
Urethritis
Bacteremia
Ophthalmia neonatorum
Filz hugh curtis syndrome
Pelvic inflammatory disease
Conjunctivitis

Question 15

Chocolate agar
Thayer martin medium(vancomycin,colistin,nystatin)
Stuarts transport medium
Charcoal impregnated swab
Above all indicate culture method of

Answer 15

N. Goorhhea
Acidify only glucose

Mueller hinton agar for n. Meningitidis

Question 16

Waterhouse fridrichsen syndrome(fulminant meningociccimia )fever,shock,multisystem failure and dissemiatedintravascular coagulation

With defieciency of C5-C9 are associated with

Answer 16

N. Meningitidis

IgA protease
Factor H binding protien
Capsular polysachharide

Question 17

Antigens of E coli

Answer 17

O somatic
H flagellar
K capsular

Question 18

Virulence factors of E coli. Antigens

Answer 18

O antigen (endotoxic ,bactericidal,phagocytosis)
KI antigen
Septicemic
F antigen
Mannose resistance hemaglutinin

Question 19

Toxins of E coli

Answer 19

Hemolysins
CNF1,siderophores
Enterolysins
Heat labile toxin(LT)
Heat stable toxin(ST)
vero toxin/shiga like toxin(VT)

Question 20

Heat labile toxin of e coli

Answer 20

SubunitA-A1 ,A2
subunit B (binding)
Activate adenylyl cyclase to firm cAMP causing watery diarrhea

Question 21

Types of Heat stable toxin of e coli

Answer 21

STA -Activates cGMP production

STB-

Question 22

Verotoxin of e coli

Answer 22

Inhibit protien synthesis

Question 23

Agar for growth of E coli (acid ,gas mr + indole+)

Answer 23

Nutrient (mucoid colony)
Blood (hemolytic)
Macconckey (fast lactose fermentor)
DCA (deoxycholate cutrate agar)
Ss ( salmonella shigella agar)
Broth  (uniform turbidity)

Question 24

Types of diarrheogenic e coli.

Answer 24

Enteropathogenic
Enterotoxigenic
Enteroinvasive
Enterogaemorhhagic
Enteroaggregative
Diffusely adherent

Question 25

4 species of shigella

Answer 25

``` Dysrnteriae Catalase - Neutoxicity,enterotoxicity,cytotoxicity Flexneri Boydii Sonnei( bacillary dysentery, late lactose fermenter) ```

Question 26

Cultural characteristics of shigella

Answer 26

XLD(xylose lysine deoxycholate) | DCA

Question 27

Antigens of salmonella

Answer 27

``` H (heat labile immunogenic, agglutinable) O (endotoxin extracted by boivin antigen/trichloroacetic acid) Vi antigen(heat labile ,acid labile , purified vaccine) ```

Question 28

Antigenic variations in salmonella

Answer 28

H to O(lost flagella, agglutinable,901 O strain) Phase variation (phase 1 species specific, phase 2 non specific converted in craiges tube) V to W (v form vi antigen agglutinate, after subcultures vi antigen is lost W form) S to R (smooth to rough, lost O antigen,virulence)

Question 29

Step ladder pyrexia is characteristic of

Answer 29

Enteric fever(salmonella)

Question 30

Rose spots on body(chest) are seen in

Answer 30

Enteric fever(salmonella) Hepatomegaly Spelomegaly

Question 31

Epidemiological types of salmonella

Answer 31

Endemic/residual typhoid | Epidemic typhoid

Question 32

Widal test is done for

Answer 32

Typhoid | Measure H and O agglutinins

Question 33

Culture media for salmonella

Answer 33

``` Macconkey(non lactose fermenter) DCA XLD(black center + H2S) Wilson and blair(b,c show black colonies) Selenite F Tetrathionate broth ```

Question 34

IMViC for salmonella

Answer 34

-+-+

Question 35

Bile broth with streptokinase test is done for clot culture of which bacteria

Answer 35

Salmonella

Question 36

Newer diagnostic tests for salmonella

Answer 36

``` IDL tubex(IgM 09 antibodies) Typhidot(IgM and IgG against 50kD antigen) ```

Question 37

TAB vaccine against O and H antigens is for

Answer 37

S. Typhi and paratyphi

Question 38

Vaccines for salmonella

Answer 38

TAB Live oral( enteric coated) Vi (live attenuated oral , Typhim Vi)

Question 39

Rice watery stools disease / cholera culture media

Answer 39

Nutrient Macconkey(lactise fermenter) Venkatraman-ramakrishnan Cary blair Monsurs taurocholate tellurite preptone water TCBS(thiosulphate citrate bile salt sucrise agar)(yellow convex colonies) BSA ( alkaline bile salt agar)