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Flashcards in bcm24 Deck (18):
1

competative

binds active site

2

non cometative

non active site bound

3

uncompetative

binds enzyme subtrate complex

4

malonate

competative on succinate

5

azt

competativve on hivrt

6

nevirapine

non competative on hivrt

7

sarine

nerve gas
group specifc on ache
destroy active site
bind tightly. permemnant modification

8

aspirin

irreversible inhibitor on cox2 enxyme
acetylates cyclooxygenase cox2 at serine in active site
prevents prostaglandin synthesis.

9

organophosphates

group specific irreversible inhibitors of acetylcholinesterase
adds phosphate to ache irreversible phosphorylation
nerve gas - sarine
insecticides - malathion

10

suicide inhibitors

substrate analogues that are activated by the catalytic cycle of the enzyme

11

suicide inhibitors examples

thymidylate synthase: dUMP->dTMP
5-F- dUMP -> 5 F dUMP destrots active site

12

competative inhibitors

often substrate analogies. like malonate to succinate

13

vmax and km of competatively inhibited enzyme

same vmax
km goes to kapp

14

linearisation of michealessmenton

1/v=km/vmax * 1/[s] + 1/vmax

15

vmax and km of competative inhibiition on lineweaver burk plot

same y intercept means same apparent vm
but higher slope means km increased to km app

16

non competative inhibitor km and vmax

km same
but vmax goes to vmax app

17

lineweaver burk plot of non competative inhibitor

higher y intercept means decreased vmax
same x intercept means same km app

18

are group specific substrate analogues

not neccessarily