BIO Q2 LQ2 Flashcards
(150 cards)
The technical application of biological knowledge for human purposes
Biotechnology
Biotechnology focuses on using living organisms like
bacteria, viruses, and fungi
Mainly used to create vaccines
Bio organisms
mainly used to create GMO Fruits and Vegetables
Plants
Mainly used to make better quality of products
Animals
Manipulation of the genetic makeup of cells or whole organisms
Genetic engineering
Universal language in biotechnology. It is present in all species.
ACTG
Cutting, splicing, and copying DNA
→ aims to amplify genetic material by combining two DNA sequences. It falls under genetic engineering.
Recombinant DNA Technology
How long is a cycle of Recombinant DNA Technology
24 hours
This is an example of _____: The process involves merging two segments of DNA strands. The first strand contains the desired DNA, such as genes from a lizard that enable wall-climbing. This desired DNA is combined with a second DNA strand from bacteria, chosen for its rapid reproduction rate. To perform this, tools are needed for cutting and pasting.
→ The lizard gene and bacterial DNA are fragmented and then joined together. As the bacteria reproduces, the desired gene multiplies. Eventually, the gene is harvested.
Recombinant DNA Technology
Human DNA is
Helical
Bacteria DNA is
Circular
Recombinant DNA technology process
- Cutting DNA into fragments
- Combining them
- Reintroducing them into
bacteria - Incubating
- Extracting and harvesting the gene
cut DNA at specific sites, often
palindromes
Restriction enzyme
Join fragments of DNA
DNA ligase
→ are strings of letters that read the same forwards and backwards.
→ They are significant because they act as signals for restriction enzymes. When these enzymes encounter palindromic sequences, they are prompted to initiate a cutting process.
Palindromic sequences
→ Cooling down the DNA sample
→ the reaction to 55-65°C for 45 seconds so primers can bind to their complementary sequences on the single-stranded template DNA
→ Allows the primer to attach to the DNA strand
→ Duration: 45 seconds
Annealing
→ Heating the reaction temperature
to 72°C for 2 minutes so Taq polymerase extends the primers, synthesizing new strands of DNA
→ Subject the DNA sample to heat again
→ Prepares for replication and the creation of complementary sister strands
→ A polymerase is added to the solution to facilitate extension
Extension
shares the same objective as recombinant DNA technology: amplifying genetic material. However, this method employs machines for a faster process, often used in medical settings for safety.
Polymerase chain reaction
How long does a cycle of PCR last?
5 minutes
PCR Cycle
- Denaturation
- Annealing
- Extension
- Cooling repeat cycle
→ Heating the reaction strongly (96°C for 1 minute) to separate, or denature, the DNA strands. This provides the single-stranded template for the next step
→ Subject the DNA to heat
→ Heat allows the hydrogen bonds to
separate, resulting in the separation of DNA strands 1 and 2
→ Duration:1minute
denaturation
Final temperature of amplicon solution should be cooling back to 5°C, this is to ensure proper bonding of newly synthesized strands of DNA
→ Cooling it down to stabilize everything
Cooling repeat cycle