Biomolecules and enzymes Flashcards
What is Le Chatlier’s Principle?
Le Chatlier’s Principle: A decrease in the concentration of compound, will lead it to shift to more of that compound
What is the role of Acetylcholinesterase? What happens if it is Inhibited?
Acetylcholinesterase: will break down Ach into Acetyl and choline.
if enzyme is inhibited, Ach levels will increase and build up
What kind of molecules can easily pass through blood-brain barrier? which are hard to pass?
Small, Hydrophobic molecules EASILY pass through blood-brain barrier (large, charged molecules have difficulty passing through )
What are trends for Michaelis Menten for Vmax, km for competitive, noncompetitive and uncompetitive ?
Competitive- vmax same, km Increases (inhibitor binds to active site and mimics substrate (analog) )
Noncompetitive; V max decreases, km same (inhibitor binds to allosteric site (another site besides active site)
Uncompetitive - vmax decreases, km decreases (inhibitor binds to enzyme substrate complex; decrease enzyme concentration )
What is Lineweaver Burk Plot? What is measured ?
Lineweaver Burk Plot (double reciprocal plot) graphical representation of enzyme kinetics
(provides more accurate estimate of vmax and more accurate info about inhibition)
y intercept— 1/ Max
x intercept - 1/km
slope–> km/vmax
(competitive: intersect along y axis, noncompetitive: x intercept stays unchanged; intersect along x axis
uncompetitive (parallel lines); mixed inhibition intersection is NOT on any of axes
How do competitive and noncompetitive inhibitors bind?
Competitive- bind reversibly to binding site
noncompetitive; irreversibly bind to binding site
What is the importance of Cholesterol?
Cholesterol: Cholesterol is an important component of plasma membranes, increasing fluidity of the membrane. It is also the precursor to steroid hormones and bile.
