Biotechnology

Question 1

What is PCR?

Answer 1

replication in a lab

Question 2

What does PCR help with?

Answer 2

solve crimes
made for studies

Question 3

What does PCR use?

Answer 3

dna primer/nucleotides, TAQ polymerase (from bacteria)

Question 4

What are the steps of PCR?

Answer 4

denature
cool DNA + add primer
reduce temp. to add bases
can repeat this cycle

Question 5

In gel electrophoresis, what pieces move faster?

Answer 5

smaller and negatively charged molecules

Question 6

What is biotechnology?

Answer 6

use of artificial methods to modify the genetic material of living organisms or cells

Question 7

What is cloning

Answer 7

The production of an exact GENETIC copy of a gene, cell, or organism

Question 8

What is gene engineering?

Answer 8

Alteration of the genetic makeup of an organism using biotechnology

Question 9

How do you do DNA fingerprinting?

Answer 9

identify a unique pattern in the genome
use a restriction enzyme
use gel electrophoresis

Question 10

What is transgenic?

Answer 10

If the foreign DNA that is introduced comes from a different species.

Question 11

What are the steps to isolate nuclei acids?

Answer 11

Note: DNA must be extracted to get nucleus acid
1. Break open cell using detergent
2. Use enzymes to destroy all undesired macromolecules
3. Avoid degradation or contamination by unsung enzymes to inactive protein, RNA, DNA, etc.
4. DNA brought to solution using alcohol. Made up of long polymers, forming gelatin mass.

Question 12

How can you reproductively clone something?

Answer 12

1. Take egg cell of one indícale and remove the nucleus
2. Take the diploid nucleus from another individual and put it into the egg cell
3. This stimulates the egg to divide