Blood Bank (cont)

Question 1

platelet concentrate preparation

Answer 1

1. Whole blood is centrifuge at 22C
   • Light spin (3200 RPM in 2-3 minutes)
2. Platelet Rich Plasma
   • Centrifuge at 22C
   • Heavy spin (3600 RPM in 5 minutes)
3. Platelets (50-70 mL) - stand for 1-2 hours at 20-24C
   • Store at 20-24C with constant agitation
   • Avoid bacterial contamination
4. Determine the weight of the plasma bag and the volume

Question 2

centrifugation of whole blood

Answer 2

22C, Light spin (3200 RPM in 2-3 minutes)

Question 3

centrifugation of PRP

Answer 3

• Centrifuge at 22C
• Heavy spin (3600 RPM in 5 minutes)

Question 4

Platelets storage

Answer 4

• Volume: 50–70 mL
• Stand for 1–2 hours at 20–24°C
• Store at 20–24°C with constant agitation
• Avoid bacterial contamination

Question 5

platelet pooling

Answer 5

• 6-8 units can be pooled in 1 bag
• shelf-life is reduced to 4 hours

Question 6

why and how is leukoreduction done

Answer 6

• to help prevent febrile non-hemolytic reactions
• done via leukoreduction filter designed for platelets

Question 7

is the liquid portion of whole blood

Answer 7

Plasma

Question 8

how is plasma prepared

Answer 8

Can be prepared from whole blood
via centrifugation or sedimentation.

Question 9

Contains all coagulation factors

Answer 9

Plasma

Question 10

Plasma is used to treat patients with?

Answer 10

liver failure, DIC, Vitamin K deficiency, warfarin overdose, single factor deficiencies, massive transfusion

Question 11

how is fresh frozen plasma prepared

Answer 11

• frozen shortly after separation from the whole blood (or within 8 hours of collection).
• 6 hours if the preservative is ACD

Question 12

Fresh frozen plasma are intended for what patients?

Answer 12

patients who are actively bleeding and have multiple clotting factor deficiencies

Question 13

Storage requirements for fresh frozen plasma

Answer 13

Stored up to 1 year after collection at - 18C or lower or stored at -65C for 7 years

Question 14

one unit of FFP contains now many plasma?

Answer 14

150-250 mL plasma

Question 15

Thawing for FFP

Answer 15

• 30–37°C water bath (bag must be covered)
• Shelf-life: 24 hrs at 1–6°C

Question 16

less factors in FFP

Answer 16

Less Factor V, Factor VII, Factor VIII, Factor X

Question 17

Uses of cryoprecipitate

Answer 17

fibrinogen replacement, Factor XIII deficiency, treatment for hemophilia A, and Von Willebrand disease

Question 18

How is cryoprecipitate prepared from fresh frozen plasma

Answer 18

• thaw FFP at 4C
• cold- insoluble proteins precipitate are collected by centrifugation at 1-6C
• supernatant is removed (via internal access port)
• 5-15 ml plasma is retained on me cryoprecipitate

Question 19

The cryoprecipitate must be refrozen within ____ of being removed from the cold environment, and stored at ___ for
up to ____

Answer 19

refrozen within 1 hour, stored at -18c or lower for 1 year

Question 20

Shelf life if cryoprecipitate is thawed

Answer 20

6 hours, or 4 hours (Pooled)

Question 21

Cryoprecipitate contains what factors

Answer 21

Factor VIII, Factor XIII, Fibrinogen, Von Willebrand Factor, and Fibronectin

Question 22

AABB requires that at least ___ of fibrinogen must be in each unit of cryoprecipitate

Answer 22

150 mg

Question 23

Storage post-thaw for cryoprecipitate

Answer 23

Room temp; transfuse ASAP (Factor VIII declines after 2 hrs)

Question 24

contents of cryo poor plasma

Answer 24

Albumin, Factors II, V, VII, IX, X, XI

Question 25

Storage for cryo poor plasma

Answer 25

Refreeze within 24 hrs, stored at -18°C for 1 year

Question 26

From plasmapheresis of donors

Answer 26

Factor VIII

Question 27

How is factor VIII acquired

Answer 27

Acquired via fractionation and lyophilisation of pooled plasma

Question 28

factor viii is intended for what patients?

Answer 28

Factor VIII deficiency patients (Hemophilia A)

Question 29

what are the other plasma derivatives

Answer 29

1. Factor IX concentrate (prothrombin complex) 2. Immune Serum Globuline 3. Normal Serum Globulin

Question 30

storage conditions for factor IX concentrate (prothrombin complex)

Answer 30

shelf life: varies on expiration date on the vial storage temp: 1-6C

Question 31

indication of Factor IX concentrate (prothrombin complex)

Answer 31

hemophilia B

Question 32

Indications of Immune Serum Globulin

Answer 32

- prophylactic treatment to patients exposed to hepatitis, measles, or chickenpox - treatment of congenital hypogammaglobulinemia

Question 33

shelf life for immune serum globulin

Answer 33

intramuscular - 3 years, intravenous - 1 year

Question 34

contents of a normal serum albumin

Answer 34

96% albumin, 4% globulin

Question 35

shelf life for normal serum albumin

Answer 35

3 years (20-24C), 5 years(1-6C)

Question 36

indications of normal serum albumin

Answer 36

plasma volume expansion (for surgery, trauma, burns)