Chap 7-9 Part 2

Question 1

What is the technique performed under sterile conditions?

Answer 1

ASeptiC

ASeptiC refers to a set of procedures aimed at preventing contamination by pathogens.

Question 2

What do we call an inanimate object or substance that can transport pathogens?

Answer 2

Fomite

Fomites can include items like doorknobs, utensils, and medical equipment.

Question 3

What is the term for the destruction of all microorganisms and their endospores?

Answer 3

Sterilization

Sterilization is a critical process in medical settings to ensure all pathogens are eliminated.

Question 4

What is the process of reducing the number of microbes in a limited area by scrubbing called?

Answer 4

Degermination

Degermination is often used in handwashing and surgical preparation.

Question 5

What is the term for introducing a small sample of a microorganism into a growth medium?

Answer 5

Inoculation

Inoculation is the first step in culturing microorganisms.

Question 6

What does the growth that appears after inoculation refer to?

Answer 6

Culture

A culture can be assessed for purity and characteristics of the microorganism.

Question 7

What are media that vary in nutrient content and consistency classified by?

Answer 7

Physical state, chemical composition, and functional type

Different classifications help in selecting the appropriate medium for microbial growth.

Question 8

What do enriched media contain to grow specific microorganisms?

Answer 8

Complex organic substances like blood or chocolate

Enriched media provide essential nutrients that certain organisms require.

Question 9

What is the most common differential stain in microbiology?

Answer 9

Gram stain

The Gram stain helps classify bacteria based on their cell wall structure.

Question 10

What color do Gram-negative bacteria appear after gram staining?

Answer 10

Red

This color change is due to the structure of their cell walls.

Question 11

What color do Gram-positive bacteria appear after gram staining?

Answer 11

Blue

Gram-positive bacteria retain the crystal violet stain due to their thick peptidoglycan layer.

Question 12

What staining method is used for microorganisms like tuberculosis that resist Gram staining?

Answer 12

Acid-fast stain

Acid-fast staining identifies bacteria with mycolic acid in their cell walls.