Flashcards in Chemistry Lab Final Deck (36):
Converting Celsius to Fahrenheit
Converting Fahrenheit to Celsius
C = (F‐32) X 0.556
Boiling point in F and C
100C and 212F
Melting point in F and C
0C and 32 F
Human body temp in C and F
37 C and 98.7 F
Room temp in F and C
20-25 C and 68-77 F
Fridge temp F and C
1-6 C and 33.8 -42.8 F
What does RCF stands for?
Relative Centrifugal Force
What is the calculation for RCF?
1.118 x 10‐5 x r x rpm2
The specimen has clotted and removed all clotting factors.
Plasma or serum?
The specimen has been kept from clotting.
Plasma or serum?
What does a lipemic specimen look like?
Top is yellow and fatty.
What must you consider for specimen collection for glucose?
If the patient is fasting
What must you consider for specimen collection for Hgb A1C?
If they are fasting?
What must you consider for specimen collection for bilirubin?
If the specimen is hemolysed
What must you consider for specimen collection for Arterial blood gas?
What type of oxygen they are on and there temperature. If the stick is arterial or venous
What must you consider for specimen collection for lipids?
If they are fasting or not?
When measuring where do you look?q
What is deionized water?
Water purified by ion exchange
What is distiller water?
Boiled and condensation gathered
What is reversed osmosis water?
Pumped through semipermeable membrane
What is ultrafiltration water?
Ultraviolet light, sterilization or ozone treatment
What is quality control procedures?
Quality control procedures are performed in a clinical laboratory to ensure that patients’ results are reliable. Reliability refers both to accuracy (how close a test is, on average, to patients' true results) and precision (the consistency of tests performed at different times).
What is the mean for testing?
Testing a healthy population
-The mean value for a control material provides an estimate of the central tendency of the distribution that is expected if method performance remains stable.
-The mean is related to accuracy or systematic error
-Any change in accuracy, such as a systematic shift or drift, would be reflected in a change in the mean value
What is standard deviation?
-is a measure of the width of the distribution
-is related to precision or random error
-Expressed as (+/‐) 1s, 2s, 3s, 4s
-standard deviation of a method often changes with concentration
-the larger the concentration, the larger the standard deviation
What is the coefficient variation?
-describes the standard deviation as a percentage of the mean.
-reflects a ratio of the standard deviation to the concentration
What are the steps for standard deviation?
Step 1:Calculate the mean of individual specimens Step 2:Calculate the difference of each specimen from the mean Step 3:Square the differences
Step 4: Add the squared differences
Step 5: Divide by the number of specimens minus one if under 20 samples
Step 6: Find the square root
How do you calculate the coefficient variation?
CV = (s/ x )100
Standard Deviation, divided by the mean, times 100
What is the formula used to make dilutions?
V1 x C1 = V2 x C2
What is the red triangle stand for and what is the levels?
0- will not burn
1- Must be pre‐heated before ignition can occur.
2- Material must be moderately heated or exposed to relatively high ambient temperature before ignition can occur.
3- Liquids and solids that can be ignited under almost all ambient temperature conditions.
4- Materials that will rapidly or completely vaporize at atmospheric pressure and normal ambient temperature, or that are readily dispersed in air and that will burn readily
What is the blue triangle and what is the levels?
0- materials that on exposure under fire conditions would offer no hazard beyond that of ordinary combustible material.
1- Material that on exposure would cause irritation but only minor residual injury.
2- Material that on intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury.
3- Material that on short exposure could cause serious temporary or residual injury.
4- Material that on very short exposure could cause death or major residual injury
What does the yellow triangle stand for and what is the levels?
0- material that in itself is normally stable, even under fire exposure conditions, and is not reactive with water.
1- Material that in itself is normally stable, but which can become unstable at elevated temperatures and pressures.
2- Material that readily undergoes violent chemical change at elevated temperatures and pressures or which reacts violently withwater or which may form explosive mixtures with water.
3- Material that in itself is capable of detonation or explosive decomposition or reaction but requires a strong initiating source or which must be heated under confinement before initiation or which reacts explosively with water.
4- Material that in itself is readily capable of detonation or explosive decomposition or reaction at normal temperatures and pressures.
What does the white triangle stand for and what is the levels?
Material shows unusual reactivity with water (i.e. don't put water on it).
OX- Material possesses oxidizing properties.
ACID- Material is an acid.
ALK- Material is a base (alkaline).
COR- Material is corrosive.
Material is radioactive
How to show professionalism?
Use good hygene
Wear clean clothes
Use professional language
Stay out of cliques
Get rest and be focused at your work station
Continue your education by staying current on changes in the field, continuing formal education, or specializing
What is beers law?
A = aλx b x c
The Beer‐Lambert law is the linear relationship between absorbance and concentration of an absorber of electromagnetic radiation. The general Beer‐Lambert law is usually written as:
where A is the measured absorbance, a is a wavelength‐dependent absorptivity coefficient, b is the path length, and c is the analyteconcentration. When working in concentration units of molarity, the BeerLambert law is written as