chpt 8: microbial growths Flashcards

(44 cards)

1
Q

study of modification of gene expression is known as

A

epigenetics

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2
Q

function of hydrogen bonds

A

provide stabilty

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3
Q

which molecule determines the parallel effect

A

sugar

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4
Q

what molecule turns to bring bases close and allows extreme stability

A

sugar

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5
Q

what is semi-conservative replication

A

1 new strand with 1 old/ parental strand

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6
Q

DNA helicase
DNA gyrase
DNA polymerase
RNA polymerase
DNA ligase
are involved in

A

semi-conservative replication

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7
Q

function of DNA helicase

A

unwinds/ unzips the helix

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8
Q

which enzyme is responsible for stabilizing unwound parent DNA

A

DNA gyrase

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9
Q

when is the replication strand formed

A

after the DNA is unwound

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10
Q

two strands of replication fork is

A
  • leading strand
  • lagging strand
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11
Q

which direction does the leading strand run from

A

5’-3’

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12
Q

function of DNA polymerase

A
  • proofread/ add new nucleotides continuously
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13
Q

which strand uses DNA polymerase

A

leading strand

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14
Q

how is the leading strand synthesized

A
  • continously
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15
Q

which strand has its direction as 3’-5’

A

lagging strand

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16
Q

what makes the lagging strand unique

A
  • synthesized in short fragments
  • not recognized by DNA polymerase
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17
Q

which enzyme in a lagging strand prepares RNA primer so the DNA polymerase can recognize it

A

RNA polymerase

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18
Q

how does DNA polymerase add nucleotides in the lagging strand

A

discontinuously in short fragments called okazaki fragments

19
Q

function of DNA ligase

A

fills in the Okazaki fragments

20
Q

info in dna is copied as RNA nucleotides sequences to produce mRNA is known as

A

transcription

21
Q

what is copied in transcription

A
  • segment of DNA known as a gene
22
Q

what is a promotor

A

site where RNA polymerase binds to DNA marking the beginning on transcription

23
Q

which DNA strand is used in transcription

24
Q

in which type of microbes does transcription occur in the nuclues

25
where does transcription occurs in prokaryotes
cytoplasm
26
what allows RNA to exit the nuclues
- single strand - RNA splicing
27
what happens in the process of RNA splicing
introns are cut away and exons join
28
function of exons
expressed region of DNA found on RNA
29
what is the unexpressed region of DNA on RNA
introns
30
which enzymes helps with RNA splicing
ribozyme
31
does RNA splicing occur in prokaryotes
no
32
function of translation
making proteins from mRNA
33
condon is
3 nucleotides that code for 1 a.a
34
AUG is
universla start codon
35
universal stop aka nonsense condons are
UAA, UAG, UGA
36
function of tRNA
carry a,a on acceptor arm
37
which part of the tRNA carries an anticodon
long arm
38
what is the site of translation
rRNA / ribosomes
39
which direction does translation occur
5'-3'
40
how does protein synthesis occur
2 subunits of ribosomes joining
41
when can translation begin before transcription is done and why
in prokaryotes cuz transcription occurs in plasma
42
what determines how a nucleotide sequence is converted to a.a
genetic code
43
how many codons code for 20 a.a
64
44
what determines the type of aminio acid that will be produced
codons on mRNA