chromatography Flashcards
(5 cards)
1
Q
explain steps in determining primary structure of a protein
A
-hydrolyse protein using HCl (NH2 groups become NH3+ so more positively charged)
-run TLC plate
-calculate Rf values and look for match on database
2
Q
why is it sometimes necessary to use two different solvents in TLC
A
to separate amino acids/substances with similar Rf values
3
Q
stationary and mobile phase for TLC
A
stationary: silica gel
mobile: non-polar liquid solvent
4
Q
stationary and mobile phase for column
A
stationary: solid silica
mobile: non-polar liquid solvent
5
Q
stationary and mobile phase for gas liquid
A
stationary: thick liquid (oil- non polar/silica) coated on column lining
mobile: inert gas
