Circoviridae

Question 1

Clinical signs of Porcine Dermatitis and Nephropathy Syndrome (PDNS)

Answer 1

Necrotizing skin lesions

Necrotizing vasculitis

Necrotizing and fibrinous glomerulonephritis

Question 2

Etiology of Chicken Infectious Anemia

Answer 2

Chicken Anemia VIrus (CAV)

Question 3

Etiology of Post Weaning Multisystemic Wasting Syndrome (PMWS)

Answer 3

Porcine Circovirus 2

Question 4

Circovirus viral DNA replication occurs in

Answer 4

the nucleus

_____________________

Requires cellular proteins and other components produced during the S phase of the cell cycle

Question 5

Common co infections associated with Post weaning multisystemic wasting syndrome

Answer 5

Porcine Parvovirus

Porcine Reproductive and Respiratory Virus

SIV

Mycoplasma hyopneumoniae

Question 6

Diagnosis of Circovirus

Answer 6

Clinical Signs

Samples

Histopathology

Serological Assays

PCV2 nucleic acids by PCR

Question 7

Diagnosis of Chicken Infectious Anemia

Answer 7

Clinical Signs

Examination of blood

Necropsy

Histopathology

Serology

Virus isolation

PCR

Question 8

Vaccination for Post weaning multisystemic wasting syndrome

Answer 8

Chimeric Vaccine - utilize the non pathogenic porcine circovirus 1 as a genetic backbone for expression of immunogenic capsid protien of PCV2

Question 9

Diseases associated with genus gyrovirus

Answer 9

Chicken Infectious Anemia

Question 10

Transmisison of Post weaning multisystemic wasting syndrome

Answer 10

Fecal-Oral Transmission

Virus found in all secretions

Vertical Transmission - Transplacental transmission

Stable and survies on fomites

Question 11

Host of Chicken Infectious Anemia

Answer 11

Highly contagious disease of young chickens

________________

2-4 weeks of age

Question 12

Distribution of post weaning multisystemic wasting syndrome (PMWS)

Answer 12

Worldwide

Question 13

Clinical signs of post weaning multisystemic wasting syndrom

Answer 13

Subclinical infection is most common

Lethargy, weight loss, cough, dyspnea, slow growth, lymphadenopathy, diarrhea, skin discoloration, congenital tremors, icterus

Question 14

Describe the genome of Genus Gyrovirus

Answer 14

Circular, Single stranded negative sense DNA

Question 15

Clinical signs of Chicken Infectious Anemia

Answer 15

Chicks are anorectic, lethargic, depressed, reduced body weight, and pale

Blood may be watery and clot slowly

PCV low

Subcutaneous hemorrhages, skeletal hemorrhages and pale muscles

Question 16

Characteristics of Porcine Dermatitis and Nephropathy Syndrome (PDNS)

Answer 16

Sporadic

Reported in older piglets

Question 17

Transmission of Chicken Infectious Anemia

Answer 17

Virus shed in feces and feather dander

Horizontal transmission through inhalation or oral exposure

Vertical transmission through egg

Environmentally stable

Question 18

Morphology of Chicken Infectious Anemia Virus

Answer 18

12 Trumpet like structures that are less obvious in other circoviruses

Question 19

Porcine Circovirus 2 primarly targets

Answer 19

Cardiomyocytes

Hepatocytes

Macrophages - fetal life

Monocytes - post natal life

Question 20

Apoptin Protein

Answer 20

Protein of Chicken Anemia Virus that induces apoptosis and cause destruction of infected lymphocytes

Question 21

Etiology of Porcine Dermatitis and Nephropathy Syndrome (PDNS)

Answer 21

Porcine Circovirus 2

Question 22

Circovirus replication occurs in

Answer 22

Actively dividing cell

Question 23

Characteristics of Family Circoviridae

Answer 23

Small viruses

Circular single stranded DNA genomes

Virions are small, nonenveloped, spherical

Question 24

Effect of transplacental infection on the fetus

Answer 24

Infection during the first and second trimesters - fetal death and resorption or aborted fetuses with severe cardiace congestion

Infection during the last trimester has minimal effect on fetus

Question 25

Principal sites of Chicken Infectious Anemia Virus replication are

Answer 25

Hemocytoblasts in the bone marrow, precursor T cells in the cortex of the Thymus and dividing CD4 and CD8 cells in the spleen

Question 26

Goal for vaccinating against chicken infectious anemia

Answer 26

Protect the progeny from vaccinated breeders from early infections by means of maternally derived antibodies

Question 27

Post weaning multisystemic wasting syndrome is characterized by

Answer 27

Individual to coalescing foci of granulomatous inflammation in lymphoid tissues, lungs, liver, kidney, heart and intestines sometime with prominent botryoid intracytoplasmic inclusion bodies

Question 28

Host of Post Weaning Multisystemic Wasting Syndrome (PMWS)

Answer 28

Pigs \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ Most common at 4-6 wees or age or 2-3 weeks post weaning

Question 29

Describe the genome of Genus Circovirus

Answer 29

Circular, single-stranded ambisense DNA

Question 30

Consistent feature in pigs that develop clinical post weaning multisystemic wasting syndrome

Answer 30

Lymphoid depletion and lymphopenia