Culture Media Preparation

Question 1

Types of Culture:

Answer 1

1. Pure Culture
2. Mixed Culture
3. Stock Culture

Question 2

Types of Culture composed of only one species

Answer 2

Pure culture

Question 3

Types of Culture composed of more than one species

Answer 3

Mixed Culture

Question 4

Types of Culture Several species contained in a separate culture medium (1 specie/culture medium)

Answer 4

Stock culture

Question 5

Growth in a large volume of broth → divide into small freezer vials (to lengthen the shelf life of specimens at least a year)

Answer 5

Stock Culture

Question 6

Used for academic and industrial purposes

Answer 6

Stock Culture

Question 7

It is composed of mixture of nutrients such as:

Answer 7

☑️ Carbon
☑️ Nitrogen
☑️ Sulfur
☑️ Phosphorus
☑️ Hydrogen
☑️ Oxygen
☑️ Buffers

Question 8

Inhibitory agents:

Answer 8

dyes, salts and antimicrobials

Question 9

is where the selection of the medium for inoculation is based.

Answer 9

Type of specimen

Question 10

If several plates will be inoculated for a given specimen, arrange starting the

Answer 10

most enriched to the most selective.

Question 11

Classification of Culture Media

Physical State

Answer 11

a. Solid Media
b. Semi-Solid Media
c. Liquid Media

Question 12

Classification of Culture Media

Classification

Answer 12

a. Synthetic Media
b. Non-Synthetic Media
c. Living Tissue Media

Question 13

Classification of Culture Media

Use

Answer 13

a. Simple Media
b. Enriched Media Differential Media
1. Differential Mildly Selective
2. Differential Moderately Selective Media
c. High Selective Media
d. Transport Media
e. Enrichment Media

Question 14

AEROBES

Answer 14

21% O2 + 0.03% CO2

Question 15

Aerobic cultures is examined after

Answer 15

18-24 hours

Question 16

ANAEROBES

Answer 16

0% 02, 5-10% CO2 + 5-10% H2 + 80-90% N2

Question 17

Anaerobic cultures required how many hours of incubation

Answer 17

48 hours

Question 18

CAPNOPHILES

Answer 18

5-10% CO2 + 15% O2 (CO2 incubator or bag; 3% CO2 in candle jars)

Question 19

MICROAEROPHILE

Answer 19

5-10% O2 + 8-10% CO2

Question 20

Most bacteria, ideal incubation temperature is

Answer 20

35°C

Question 21

Microaerophile which has reduced O2 and increased CO2

Answer 21

Campylobacter and Helicobacter

Question 22

Uses 42°C for optimum growth

Answer 22

Campylobacter and Pseudomonas

Question 23

COOK → AUTOCLAVE → DISPENSE

Tube Medium

Answer 23

Nitrate (Slant),
Malonate,
MIO

Question 24

COOK → AUTOCLAVE → DISPENSE

Plated Medium

Answer 24

BA, CA, MAC, PEA, MHIA Plain,
MHIA with Blood,
MHIA with
Nal, Cornmeal,
SDA Plate, NA

Question 25

AUTOCLAVE → COOK → DISPENSE Tube Medium

Answer 25

TSI, TSB, Tryptone, TSA, Tryptone, Thio SC, SDB, SCA, PAF, PAD, PAD, NSS, LIA, BEA, ATM, 6.5% NaCi, LSB, EC broth, BGLB, Sugars

Question 26

AUTOCLAVE → COOK → DISPENSE Plated Medium

Answer 26

SSA, TCBS

Question 27

COOK → DISPENSE → AUTOCLAVE Tube Medium

Answer 27

Urease, TETRA broth, Selenite broth

Question 28

MACCONKEY AGAR

Answer 28

1. Refer to Table 2.2 (C-A-D) 2. Mix agar, 5g MAC to 100 mL distilled water. Boil with continuous swirling. 3. Autoclave at 121°C. 4. Dispense onto sterile plates. Avoid formation of air bubbles.

Question 29

BLOOD AGAR

Answer 29

1. Refer to Table 2.2. (C-A-D) 2. Mix agar, 4g TSA to 100 mL distilled water. Boil with continuous swirling 3. Autoclave at 121°C. (15-30 mins) 4. Cool to 50°C. Add 5 mL blood aseptically and mix gently. → common error in laboratory 5. Dispense onto sterile plates. Avoid formation of air bubbles

Question 30

CHOCOLATE AGAR

Answer 30

1. Refer to Table 2.2 (C-A-D). 2. Mix agar, 4g TSA to 100mL distilled water. Boil with continuous swirling 3. Autoclave at 121°C. Add the 5mL blood aseptically while hot and mix gently. 4. Dispense onto sterile plates. Avoid formation of air bubbles.

Question 31

MUELLER-HINTON PLAIN AGAR

Answer 31

1. Refer to Table 2.2 (C-A-D). 2. Mix agar, 3.8g of MHIA to 100mL distilled water. Boil with continuous swirling 3. Autoclave at 121°C. 4. Dispense onto sterile plates. Avoid formation of air bubbles

Question 32

NUTRIENT AGAR

Answer 32

1. Refer to Table 2.2 (C-A-D). 2. Mix agar, 2.3g of Nutrient Agar to 100mL distilled water. Boil with continuous swirling. 3. Autoclave a t 121°C. 4. Dispense onto sterile plates. Avoid formation of air bubbles.