POCT: IMMUNOCHROMATOGRAPHIC TEST FOR HBsAg

Question 1

It is a complex DNA virus belonging to the family Hepadnaviridae, with its members collectively known as hepadnaviruses.

Answer 1

hepatitis B virus (HBV)

Question 2

called “serum hepatitis,”

Answer 2

Hepatitis B

Question 3

Hepatitis B viral proteins of significance include

Answer 3

● Envelope protein - Hepatitis B surface antigen (HBsAg)
● Structural nucleocapsid core protein - Hepatitis B core antigen (HBcAg)
● Soluble nucleocapsid protein - Hepatitis B e antigen (HBeAg)

Question 5

HBV spreads through

Answer 5

infected body fluids such as
- blood,
- saliva,
- semen,
- vaginal secretions,
- tears,
- breast milk,
- sweat,
- urine.

Question 6

HBV mode of transmission

Answer 6

● Sharing of contaminated needles or syringes
● Blood transfusion
● Sexual intercourse with an HBV-infected person
● Transmission from an HBV-infected mother to her newborn baby

Question 7

DENGUE

Answer 7

For detection of IgG/IgM
- Dispense 5uL of specimen to sample well (S well)
- Add 3 drops of buffer (B well)
- Set timer

For detection of dengue antigen
- Dispense 2 drops serum sample
- Add 1 drop buffer (sample diluent) to S well
- Set timer
- Read at 20-25 mins

Question 8

SYPHILIS

Answer 8

Add 2-3 drops (80 uL) on sample area
Read at 15 mins

Question 9

ASO

Answer 9

Add 1 drop of sample to the first well
Add 1 drop of positive and negative controls on middle and last well, respectively
Add 1 drop of ASO latex to each well
Mix using paddle end of pipette
Gently tilt and rotate slide by hand for 2 mins
Observe any agglutination

Question 10

INFLUENZA

Answer 10

Perform throat swab on patient’s pharynx and tonsillar area
Insert absorbent tip of swab to extraction buffer, swirl the swab 5 times
Put the nozzle on extraction tube
Add 3 drops of specimen to the sample well of the cassette
Read at 15 minutes