PIPETTING TECHNIQUES Flashcards
It holds a particular volume but does not dispense the exact volume.
To Contain (TC)
Also known as rinse-out pipette
To Contain (TC)
Ex of To Contain (TC)
Sahli hemoglobin and Long Levy Pipettes
It delivers the exact amount it holds into a container; should not be blown out
To Deliver (TD)
It is designed to drain by gravity, and the desired volume is obtained when draining stops.
To Deliver (TD)
Ex of TD
Serologic, Mohr, and Volumetric transfer pipettes.
It has continuous etched rings on top or near the mouthpiece of the pipette and exact volume is obtained when the last drop is blown out.
Blow-out Pipette
This type of pipette is not rinsed out.
Blow-out Pipette
2 continuous rings ➡for viscous fluids
Blow-out Pipette
Ex of Blow-out Pipette
Ostwald-Folin and Serologic Pipettes
It allows the liquid to drain by gravity
Self-draining Pipettes
It does not have etched or frosted rings.
Self-draining Pipettes
For non-viscous fluids
Self-draining Pipettes
Measuring or Graduated
- Serologic
- Mohr
- Bacteriologic
- Ball, Kolmer, or Khan
- Micropipettes (TC pipettes/Glass Micropipettes)
Transfer
Volumetric
Ostwald-Folin
Pasteur Pipette
Automatic macropipettes or micropipettes
With graduations to the tip
Blow-out pipette
Serologic
Without graduations to the tip
Calibrated between 2 marks
Self-draining pipette
Mohr
For nonviscous fluid
The bulb is at the middle
Self-draining
Small amount left in the tip should not be blown out
Volumetric
The bulb is near the mouthpiece
For viscous fluid ➡ that is greater than water
With etched ring
For blowout
Ostwald-Folin
Transfer fluids without consideration of a specific volume
No calibration marks
Pasteur Pipette
