CyTOF Flashcards Preview

Techniques Course > CyTOF > Flashcards

Flashcards in CyTOF Deck (8):
0

Basic steps in cytof

Sample introduction
Ionization
Mass analysis
Data acquisition

1

How is the plasma created?

Using a radio-frequency-generated electromagnetic field in the plasma torch

2

3 chambers of the torch

Outer - argon plasma gas
Central - argon auxiliary gas
Inner - in the sample injector (transmits argon stream and sample aerosol from spray chamber to center of plasma)

3

Things found inside the ion beam

Heterogenous mixture if argon ions, endogenous cellular ions, isotopic probe ions, neutral particles and photons

4

Role of interface region

- reduces temp and pressure of incoming clouds
- focuses the ionic beam to the downstream ion optics
- accelerated and highly focused ion beam after the reducer

5

Role of quadrupole

Removes unwanted low MW argon and endogenous cellular ions

6

What does cytof signal depend on.

Target abundance
Probe performance
Instrument detection efficiency for the metal isotope tag

7

Why use cytof?

- can stain up to 40 markers
- reduces the quantity of samples needed
- allows use of multiple tetramers
- can barcode cells