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Flashcards in Protein Fluorescence Deck (8):
0

What is fluorescence

The light emitted by an atom or molecule after a finite duration subsequent to the absorption of electromagnetic energy

1

Why do we use fluorescence

Provides info on molecular environment and on dynamic processes

2

Things that can be measured with fluorescence

- temp
PH
Viscosity
Ions
Polarity

3

Instrumentation in fluorescence

Fluorimeters
Microscopes
High throughout plate readers
Intravital imaging

4

What is the dominant intrinsic fluorophore?

Tryptophan

5

3 ways to make a GFP fusion construct

Protein x - GFP
GFP - protein y
Protein -GFP - z

6

Purpose of FRET

Fluorescence resonance energy transfer
Allows the detection of molecule-molecule interactions in the nanometer range with light microscopy

7

MDCK clues

Madin-Darby canine kidney polarized epithelial cells