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Flashcards in Cell Culture Deck (3):

Types of culture and cell lines

Primary cells
Continuous lines
Transformed cells
Hybridomas (fusion of lymohocytes and immortal cells)


Classification by manner of growth

- adherent growths
- suspension cultures
- hanging drops


How to sub-culture

- remove media and treat cells with trypsin and EDTA
- inactivate trypsin with medium-containing serum
- excess protein titrates away protein
- cells vigorously re suspended in fresh medium and plated