Protein Purification: Chromatography Flashcards Preview

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Flashcards in Protein Purification: Chromatography Deck (5):
0

Types of chromatography

Affinity
Ion exchange
Hydrophobic interaction
Gel filtration

1

Equipment for liquid chromatography

- refrigeration
- buffer reservoir
- pump and gradient maker
- way to apply protein sample to column
- detection and monitoring system
- fraction collector

2

Definition of amphoteric

Net surface charge changes as pH changes

3

Steps in gradient elution

Equilibration
Sample injection volume
Gradient elution
Wash
Re-equilibration

4

What is kav?

A partition coefficient related to the size of a molecule

Globular proteins have a partially linear relationship between kav and logMr