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Flashcards in Protein Methods Deck (3):

Value of protein purification

Can establish particular biological activity
Produce valuable biochemical reagents
Understand enzyme mechanisms and inhibitors
Create novel protein
Understand 3d structure


Requirements for protein purification

An assay for the protein of interest
A reasonable source of that protein


Basic scheme of protein purification

- prep, extraction, clarification
- capture
- intermediate purification
- polishin