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Flashcards in Protein Methods Deck (3):
0

Value of protein purification

Can establish particular biological activity
Produce valuable biochemical reagents
Understand enzyme mechanisms and inhibitors
Create novel protein
Understand 3d structure

1

Requirements for protein purification

An assay for the protein of interest
A reasonable source of that protein

2

Basic scheme of protein purification

- prep, extraction, clarification
- capture
- intermediate purification
- polishin