Dental Tissue Regeneration Flashcards
(22 cards)
name the labile (continuously dividing) cells in tissues?
stem cells
skin epithelium
salivary gland
GI epithelium
name the stable (quiescent) cells in tissues?
fibroblasts
endothelial cells
smooth muscle cells
lymphocytes
name the permanent (non-dividing) cells in tissues?
cardiac muscle cells
skeletal muscle cells
what type of cells are the main actors in regeneration?
stem cells
list different types of stem cells?
embryonic stem cells
induced pluripotent stem cells
adult stem cells
muscle stem cells
in regeneration, what can mesenchymal stem cells differentiate in to?
osteoblast (bone)
chondrocytes (cartilage)
adipocyte (fat)
how do we get the dental stem cell?
- tooth placed in transport medium immediately after exodontia/ exfoliation
- separation of dental tissue containing stem cells
- explant or enzymatic and/ or mechanical dissociation of dental tissue
- dental stem cells in culture
what are the goals of pulp regeneration?
histological reconstruction of the pulpodentinal complex
clinical recovery - resolve clinical symptoms, return physiological functions, further root maturation
list pulp regeneration strategies using DPSCs?
DSPSc/SHED sheets/ aggregates/ pellets
DPSCs/ SHED organic/ synthetic scaffolds
DPSCs/SHED pretreated/ combined with growth factors
DPSCs/ SHED together with endothelial cells
explain the bone regeneration approach using DPSCs?
dental pulp is extracted from human teeth
DPSCs are expanded under specific conditions
DPSCs are seeded on collagen scaffold
scaffolds with DPSCs are implanted in animal models
after 30-60 days bone tissue is recovered from animals
in the first study in human for bone regeneration using DPSCs, how long did it take for bone to regenerate?
3 years after graft
what is the role of platelet rich fibrin in bone regeneration?
regenerative additive currently used in dentistry
acts as a bioactive surgical additive to induce wound healing
used in maxfax to enhance stem cells for bone regeneration in the host bone and bon graft
could serve as a resorbable membrane for guided bone regeneration
what has been used for dental papilla regeneration?
scaffold - hylauronic acid
stem cells - BMMSCs
growth factors - PRP preparation
what are stable (quiescent) cells?
they dont do anything until required
what are liable cells for?
regeneration
what happens if injury occurs to permanent cells?
scar tissue
what do we need to engineer tissues?
cells
biomaterial scaffold
growth factors/ bioreactor
what can stem cells do?
self renew to form more stem cells
differentiate to a specialised cell
advantages of adult stem cells?
easily obtained in large number
safe to implant
able to differentiate into cells needed
what is the role of PRF in bone regeneration?
Scaffold
stimulates stem cells to migrate to the site
what gene is responsible for hypodontia?
USAG1
