Diagnostic Role of the Blood Film Flashcards
What is the H&E stain
Haematoxylin and Eosin stain
Haematoxylin
Purple blue that binds to acidic compounds including DNA and shows up the nucleus
Eosin
Pinkish stand that binds protein components in the cytoplasm
How to investigate for a specific molecular component
Detect the different refractive index of cells compared with surrounding fluid
How to use a light microscope
Focus the image Focus the condenser (close the field iris) and adjust the condenser until you get a sharp image of the edge of the disc of light coming through iris Adjust field iris and stop when whole field is only just illuminated Adjust the condenser iris by opening it and closing it until the image begins to darken
What is leishman stain
Purple blue dye that stains nuclei
How to stain blood with leishman
Slide is placed blood-side up over draining dish Leishman stains are dropped using dropper bottle Slide is left to stain Buffer is added Stain is poured off and rinsed well\ Slide is dried
Cell type
Reference ranges
Derived from a reference population
Normal range
Less strict
Derived from a healthy reference population
What are important points when interpreting laboratory data
Value within normal range may be abnormal for that individual
Value outside the normal range may be normal - 5% of healthy subjects
Reference ranges for healthy and sick individuals usually overlap
Use reference range derived fgor a particular instrument
What is an elevated number of eosinophils responsible for
What is an elevated level of basophils indicative of
Chronic myelogenous leukemia
Hypersensitivity reactions
Do you think allergies or hypersensitivity reactions are likely to explain the elevated numbers of eosinophils and basophils in a cohort of medical students
What is this indicative of
Two band form neutrophils
Indicative of left shift
Increase in proportion of band cells
Prescence of clear vacuoles
What is this indicative of
Acute lymphoblastic leukaemia
Irregular shape of the nuclei
What is this indicative of
Chronic myelogenous leukaemia
Granuloycytic cells from myeloblasts (left shifts)
What is this indicative of
Megaloblastic anaemia
Maturation of the nucleus is retarded in relation to cytoplasm
Hypersegmentation
What does a FBC include
