DNA replication (I2I) Flashcards
(23 cards)
DNA replication
Production of two identical DNA molecules from one original template molecule.
DNA replication take place after cell division.True or False?
False
before cell division (s phase)
DNA replication takes place during _____ of cell division.
S phase (synthetic phase)
DNA replication is ______.Why?
semiconservative
bcus new made strands contain single strand of template DNA.
DNA replication takes place on ______ of chromosomes.
Origin of replication
Types of DNA in prokaryotes and its origin of replication
Circular DNA
Only one origin of replication
Types of DNA in eukaryotes and its origin of replication
Linear DNA
Thousands of origin of replication
DNA replication directs ______ the replication fork.
towards the replication fork
The template strands must be separated in order to replicate.True or False?
True
Replication takes place on both strands simultaneously.True or False?
True
Replication takes place from every origin of replication at the same time in eukaryotic cell.True or False?
True
Enzyme that separates two strands of template DNA
Helicase
Function of Helicase
separate two strands of template DNA
Function of single strand binding proteins
Stablizie the unwound template strands
Function of topoisomerase
Relieve super coiling by breaks and rejoins the strand ahead the replication fork.
(DNAရဲ့backboneမှာရှိတဲ့phosphodiester bondကိုဖျက်ပြီးပြန်ဆက်ပေး)
Enzymes that synthesize DNA
DNA polymerase 3
Function of DNA polymerase 3
to synthesize new DNA strands by adding nucleotide
Can DNA polymerase 3 initiate replication by itself?
No
it need primer in order to initiate replication.
Function of primase
to synthesize RNA primer in order to initiate replication
Nucleoride of Primer
RNA primer -Ribonucleotide
DNA pol 3 can synthesize new strands only in ______ direction.
5’ to 3’ direction
(3’ OH endကိုပဲnucleotides အသစ်တွေပေါင်းပေးနိုင်)
Replication direction according to newly synthesized strand
5’ to 3’ direction
Replication direction according to template strand
3’ to 5’ driectionကိုပဲဖတ်ပြီး အသစ်တွေထည့်သွား