DNA sequencing comparison Flashcards Preview

GN 421 > DNA sequencing comparison > Flashcards

Flashcards in DNA sequencing comparison Deck (15):
1

Types of sequencing

- Sanger-automated
- Pyrosequencing
- Illumina
- Ion Torrent
- Pac Bio
- Nanopore

2

genomic library cloned into plasmids

- Sanger automated

3

uses chain termination

- Sanger automated
- Illumina

4

uses DNA synthesis

- Sanger-automated
- Pyrosequencing
- Illumina
- Ion Torrent
- Pac Bio

5

Requires a genomic library

- Sanger-automated
- Pyrosequencing
- Illumina
- Ion Torrent
- Pac Bio

6

involves massively parallel sequencing

- pyrosequencing
- illumina
- ion torrent
- pac bio
- nanopore

7

measures the release of pyrophosphate

- pyrosequencing

8

measures light production

- pyrosequencing

9

is the most expensive to sequence an entire genome

- pyrosequencing

10

best for highly repetitive DNA

- Pac Bio

11

measures the release of H+ ions

- Ion torrent

12

generates paired end reads

- Illumina

13

most likely used in the clinic

- nanopore

14

used for personal sequencing

- Pyrosequencing

15

real time single molecule sequencing via movie production

- Pac Bio