Structure of Genome Part 3 Flashcards Preview

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Flashcards in Structure of Genome Part 3 Deck (15):
1

renaturation faster for

- DNA molecules at higher concentration
- easier to find complementary base pairs

2

rate of renaturation

- inversely proportional to the complexity of the reassociating DNA

3

complexity

- length of unique sequence DNA
- repeated sequences add to the complexity only once no matter how often they are repeated

4

cot curves

- Curves that examine the renaturation of DNA and the kinetics of renaturation

5

if two genomes are different in size (on a bp per genome basis or a mass per genome basis) that when the two genomes are present in the same concentration on a MASS PER VOLUME BASIS.

- the smaller genome will be more concentrated on a MOLECULES OF DNA PER VOLUME BASIS.

6

two genomes of different sizes are present at the same concentration on a MOLECULES OF DNA PER VOLUME BASIS

they will renature at the SAME RATE.

7

- Co stands for

-units of Co

- t stands for

- concentration of DNA on a mass/volume basis

- ug/ml or a moles bp/liter.

- time of reassociation (usually in seconds)

8

Cot1/2

- a specific cot at which half the renaturing molecules are single-stranded and half are double-stranded
- the Cot when renaturation is half complete

9

Does knowing the amount of DNA on a mass per volume basis tell us anything about the size of the DNA molecule?

No; independent of of the size of the molecule

10

Cot1/2 for any specific genome or group of DNA molecules is

- directly related to the complexity of the DNA in genome

11

Cot 1/2 is a characteristic of any genome or group of DNA molecules

- does not depend on concentration

12

units for Cot

(ug*sec)/mL

13

How would the Cot½ for E. coli DNA carried out at 1 ug/ml compare to the Cot½ of E. coli DNA carried out at 10 ug/ml?

it would be the same

14

If two genomes of different sizes are present at the same concentration on a MASS PER VOLUME BASIS

- the smaller genome will renature more rapidly.

15

concentration in terms of cot

- on a MASS PER VOLUME BASIS