Flashcards in Structure of Genome Part 3 Deck (15):
renaturation faster for
- DNA molecules at higher concentration
- easier to find complementary base pairs
rate of renaturation
- inversely proportional to the complexity of the reassociating DNA
- length of unique sequence DNA
- repeated sequences add to the complexity only once no matter how often they are repeated
- Curves that examine the renaturation of DNA and the kinetics of renaturation
if two genomes are different in size (on a bp per genome basis or a mass per genome basis) that when the two genomes are present in the same concentration on a MASS PER VOLUME BASIS.
- the smaller genome will be more concentrated on a MOLECULES OF DNA PER VOLUME BASIS.
two genomes of different sizes are present at the same concentration on a MOLECULES OF DNA PER VOLUME BASIS
they will renature at the SAME RATE.
- Co stands for
-units of Co
- t stands for
- concentration of DNA on a mass/volume basis
- ug/ml or a moles bp/liter.
- time of reassociation (usually in seconds)
- a specific cot at which half the renaturing molecules are single-stranded and half are double-stranded
- the Cot when renaturation is half complete
Does knowing the amount of DNA on a mass per volume basis tell us anything about the size of the DNA molecule?
No; independent of of the size of the molecule
Cot1/2 for any specific genome or group of DNA molecules is
- directly related to the complexity of the DNA in genome
Cot 1/2 is a characteristic of any genome or group of DNA molecules
- does not depend on concentration
units for Cot
How would the Cot½ for E. coli DNA carried out at 1 ug/ml compare to the Cot½ of E. coli DNA carried out at 10 ug/ml?
it would be the same
If two genomes of different sizes are present at the same concentration on a MASS PER VOLUME BASIS
- the smaller genome will renature more rapidly.