Enterics

Question 1

Enteric properties (gram, oxidase, growth, fermentation, reduction, motility)

Answer 1

Gram negative bacilli
Oxidase negative
facultative anaerobes
ferments glucose = acid and gas (aerogenic) OR acid only (anaerogenic)
reduce nitRATES to nitRITES
peritrichous flagella or non motile

Question 2

Which is the only oxidase pos enteric

Answer 2

Plesiomonas

Question 3

where are isolation of enterobacters are significant

Answer 3

extraintestinal origin (urine, wound, swabs)

Question 4

which specimens are specifically examined for enterics

Answer 4

fecal specimens from intestinal disease patients

Question 5

what is an enteric screen?

Answer 5

combination of differential, selective, and enrichment media chosen to isolate organisms

Question 6

what organisms lead to acute gastrointestinal disease

Answer 6

bacillus cereus, staph aureus, clostridium perfringins

Question 7

how does the GI become distressed due to organisms?

Answer 7

intoxication

Question 8

what is the appearance of salmonella, shigella, and yersinia on MAC

Answer 8

wt

Question 9

what is salmonella on hektoen

Answer 9

blue-green with black colonies

Question 10

what is shigella on hektoen

Answer 10

green transparent

Question 11

what is yersinia on hektoen

Answer 11

blue-green

Question 12

what does yersinia look on cefsulodin irgasan novobiocin (CIN)

Answer 12

pink-red bulls eyes

Question 13

what does Ecoli O157:H7 look on SORB/SMac and STEC

Answer 13

colorless on SMac

mauve on STEC

Question 14

what is the plating for spot indole

Answer 14

BAP/CAP

Question 15

what is the reagent for kovacs indole

Answer 15

dimethylamino benzaldehyde

Question 16

hat is the reagent for spot indole

Answer 16

p-dimethylaminocinnaldehdye

Question 17

purpose of CNA and PEA aerobic incubation?

Answer 17

CNA = GP bacteria isolation (and yeast)

PEA = selects GPC staph and strep species (isolation of facultative anaerobes***)

Question 18

What 2 conditions are needed for decarboxylation

Answer 18

anaerobic and acidic condition (37 degrees)

Question 19

why do we need a control tube for decarboxylation

Answer 19

tests if amino acids are used or not by the organism

Question 20

what is STEC media

Answer 20

qualitative media to detect shiga-like toxin producing Ecoli

Question 21

when do we use susceptibility testing on enterics

Answer 21

immunocomp and if treatment is not working

Question 22

when do we report yeast in stool

Answer 22

predominant organism present

Question 23

what bacteria grows pk on MAC

Answer 23

Ecoli (pink) and O157:H7
Kleb pneumo
Klab oxytoca
Kleb aerogenes
Enterobacter cloacae
Citrobacter frundii

Question 24

what is the TSI of a pk MAC

Answer 24

A/A g

Question 25

which bacteria is pk on MAC ONLY with A/A g and H2S

Answer 25

citrobacter freundii

Question 26

which bacteria is wt on MAC ONLY with A/A g and H2S

Answer 26

proteus vulgaris

Question 27

what is the TSI of wt on MAC

Answer 27

K/A g

Question 28

what are the bacteria with wt on MAC

Answer 28

Ecoli (white) proteus mirabilis proteus vulgaris providencia rettgeri morganella morganii salmonella shigella yersinia

Question 29

what is the TSI of yersinia

Answer 29

A/A sick (orange bottom, red-yellow top)

Question 30

what color is serratia marcescens on MAC

Answer 30

cherry red

Question 31

what does non fermenters mean

Answer 31

aerobic, GNB, that cannot use carbs

Question 32

are NFB opportunistic agents on debilitated hosts (T/F)

Answer 32

T

Question 33

where is burkholderia most commonly seen

Answer 33

CF patients

Question 34

what are the NFB?

Answer 34

pseudomonas, steno, burkolderia, alcaligenes, moraxella lacunata, acintobacter

Question 35

which NFB shows no grow on MAC

Answer 35

moraxella lacunataa

Question 36

which NFB is wet mount negative

Answer 36

moraxella lacunata and acinetobacter

Question 37

what are the neg motile enterics

Answer 37

kleb pnuemo, ocytoca shigella yersinia