Quality control and Susceptibility Flashcards
what is quality assurance?
- monitor ongoing total tests and effectiveness
- correct problems to ensure accurate results
- assess competency of staff (pre, post, analytical)
what is quality control
monitor quality of each method to ensure accuracy and reliability
what are examples of benchmarking
- reference point
- seeking best practices to imitate
- done willingly
- productivity and cost
what does CLSI stand for
clinical and laboratory standards institute
what does NCCLS stand for
national committee for clinical laboratory standards
what is the CLSI vision
leader in clinical and lab standards to improve quality of care
what is CLSI mission
develop best practice in lab in the wold
what is CLSI values
excellence, responsiveness, inclusiveness, fairness
what is ATCC and what do they do
american type culture collection
- global supply of standard strains of typical morphs and biochem characteristics
what are the routine schedule components of a QC program
mechanical
media
reagents
antisera
antimicrobial disksw
when should incubators and fridges be cleaned inside
every 3 months
when should freezers be defrosted
every 6 months
where are chemical indicators seen
monitoring temperature and pressure of autoclaves
when should biological spore strips be performed on autoclaves
every month
what are the chemical controls in anaerobe/CO2 conditions
methylene blue for CO2
resazurin for anaerobes
fyrite CO2 testing
what are biological controls in anaerobic and CO2 conditions
Clostridium haemol - strict anaerobe
Neisseria gonnorrh - needs CO2
Campy jejuni - microaerophilic
how many plates undergo sterility testing
2-5% of final product are test plates for sterility
<100 plates received = all plates tested for sterility
what is sterility testing
media is placed in temperatures for 24 hours when those temperatures are needed for testing
how is pH of plates tested
surface electrodes or macerate the media and test at RT
does the pH get affected by sterilization T/F
T
what is performance testing for plates
nutrient quality and reliability using heavy inoculum
what are macroscopic tests for media before use
clairty and color
moisture free
no signs of dryness
how should reagents be stored
closed brown or opaque containers
-20 to 20 degrees
how long is short term storage for antimicrobial disks
1 week
where are long term antimicrobial disks stored
in freezer
what is required for each antimicrobial kit and why
desiccant to prevent moisture
how does an internal proficiency testing work
supervisor introduces unknown specimens to regulate the workflow and check for errors
purpose of internal proficiency testing
check sensitivity of routine methods to detect pathogens and staff ability to isolate
how does external proficiency testing work
rare and unusual samples received from outside associations are run and then compared by supervisors
what is the purpose of external proficiency testing
determines lab ability to ID difficult organisms - accuracy
who is the source of stock cultures
American type culture collection (ATCC)
how is semi stock cultures prepared
- lyophilized vial is subbed to media.
- second subbing is inoculated to freezer vials.
- new vial of same organism removed to prevent repeated thawing and freezing (prevent mutations)
