Enzyme Kinetics 1 Flashcards
(8 cards)
1
Q
What happens as conc of substrate increases ?
A
The fraction of enzyme molecules bound to substrate increase, so they build the product faster
2
Q
What is the role of B-Galactosidase ?
A
To hydrolyse lactose to form galactose and glucose
It can also hydrolyse Nitrophenylgalactose
3
Q
What is the Michaelis-Menten equation ?
A
See on paper
4
Q
What is Km ?
A
Michaelis constant
The substrate concentration at which v=Vmax/2
5
Q
What is rate of formation of [P] proportional to ?
A
[ES]
6
Q
What does Kcat describe ?
A
How fast an enzyme works
7
Q
What does Km describe ?
A
An enzymes substrate concentration dependence
8
Q
What is the best measure of an enzymes catalytic efficiency ?
A
Kcat/Km
For a good enzyme:
Kcat=high
Km=low
Kcat/Km=high
