enzymes 4

Question 1

wat doen proteasen?

Answer 1

Proteasen verbreken de peptidebindingen tussen twee aminozuren door middel van het toevoegen van een watermolecuul: hydrolyse. Hydrolyse van eiwitten wordt proteolyse genoemd.

Question 2

hoe wordt de hydrolyse van een eiwit genoemd?

Answer 2

proteolyse

Question 3

wat doet protease?

Answer 3

cuts the peptide bond

Question 4

aminopeptidase cuts from

Answer 4

H2N

Question 5

carboxypeptidase cuts from the

Answer 5

COOH

Question 6

Chymosin (rennin) is added to milk during production of cheese and does two things:

Answer 6

1. coagulation of milk protein
2. contributes to flavour and texture

Question 7

other applicaition of proteases

Answer 7

1. beer
2. baking
3. meat
4. protein hydrolysis/ modification

Question 8

why is chymosin the preferred enzyme for cheese production?

Answer 8

Chymosin specifically cleaves κ-casein into para-
κ-casein

Question 9

what does lipase do?

Answer 9

converts triglyceride in –> glyceride + fatty acids

Question 10

where is lipase active?

Answer 10

oil/ water interface

Question 11

what does lipoxygenase do?

Answer 11

conversion of fatty acids with a cis, cis, 1-4-pentadiene system into instable hydroperoxides

Question 12

what are the differences between lipas and lipoxygenase?

Answer 12

• Hydrolase versus oxidoreductase
• Cleaving at glycerol-fatty acid ester-bond versus O2 addition to fatty acid chain forming instable hydroperoxides.