Eukaryotic Transcription

Question 1

All protein-coding genes, plus snoRNA genes, miRNA genes, siRNA genes and most snRNA genes are transcribed by what enzyme

Answer 1

RNA polymerase II

Question 2

tRNA genes, 5S rRNA genes, some snRNA genes and genes for other small RNAs are transcribed by

Answer 2

RNA polymerase III

Question 3

5.8S, 18S, and 28S rRNA genes transcribed by

Answer 3

RNA polymerase I

Question 4

Three conserved sequences of promoters in eukaryotic cells

Answer 4

1) a TATA (hogness) 25 (upstream)
2) a CAAT 70 (upstream)
3) GC-boxes -40 and -110

Question 5

Basal transcription complex (made of) which binds to Inr (promoter region)

Answer 5

RNA polymerase and transcription factors

Question 6

Basal transcription complex binds to (to begin transcription)

Answer 6

Promoter (Inr) that containing conserved sequences

Question 7

Basal transcription complex involves both

Answer 7

BTE (elements)

BTF (factors)

Question 8

BTEs include

Answer 8

TATA-box (25)
CAAT-boxes (70)
GC-rich (-40 to -110)

Question 9

BTFs are considered

Answer 9

Minimal proteins complement (needed for accurate transcription)

Question 10

Transcription regulatory elements

Answer 10

Upstream
Downstream
Within genes
Enhance (polymerase activity)
Silence (polymerase activity)