Prokaryotic Transcription

Question 1

Core enzyme of prokaryotic polymerase

Answer 1

4 units (a2BB-)

Question 2

What is required for initiation of RNA synthesis in prokaryotes?(

Answer 2

Fifth subunit (sigma factor)

Question 3

In prokaryotic cells, RNA polymerase binds to DNA with high affinity to

Answer 3

Promoter region

Question 4

The promoter region in prokaryotic cells is located where in relation to the gene start?

Answer 4

Upstream

Question 5

What in the promoter region of prokaryotic cells regulates gene transcription?

Answer 5

Conserved sequences (signals)

Question 6

Determines the start, frequency and direction of RNA synthesis

Answer 6

Signals (conserved sequences)

Question 7

Mediates binding of RNA polymerase to promotor region in prokaryotic cells

Answer 7

Sigma factor

Question 8

RNA polymerase is responsible for breaking down H+ bonds in order to

Answer 8

Unwind DNA

Question 9

In prokaryotic transcription, what is the first to be incorporated (purine or pyrimidine)?

Answer 9

Purine

Question 10

Stage of transcription that involves sigma factor

Answer 10

Initiation

Question 11

Process of unwinding DNA (downstream), sequential polymerization (joining) of nucleotides and rewinding DNA (upstream)

Answer 11

Elongation

Question 12

Process in which elongation ends

Answer 12

Termination

Question 13

Termination of transcription in prokaryotic cells continues until what is received

Answer 13

Termination signals

Question 14

In E. coli, transcriptional termination occurs by what two means

Answer 14

Factor-dependent

Factor-independent

Question 15

Formation of stem-loop in the RNA destabilizes the association between the polymerase and the DNA template

Answer 15

Factor-independent termination

Question 16

Depends on the presence of two symmetrical GC-rich segments capable of forming a stem-loop structure in the RNA

Answer 16

Factor independent termination

Question 17

Requires the recognition of termination sequences by the termination protein, known as rho (p) factor

Answer 17

Factor-dependent termination

Question 18

Binding of rho factor destabilizes the polymerase-template interaction and results in dissociation of the polymerase and termination transcription

Answer 18

Factor-dependent termination

Question 19

mRNA degraded in minutes in this type of prokaryote

Answer 19

E. coli

Question 20

rRNA is cleaved prokarytic cells to produce

Answer 20

70S

23S and 5S > 50S (34 proteins)
16 S > 30S (21 proteins)

55 proteins