Ex. 11 Biochemical Testing for Identification Flashcards
What does Urease test for?
An organisms ability to produce the enzyme urease.
________ is a waste product of animal metabolism.
Urea
If Urea is present in the Urease study it will produce ________ and _________
ammonia and CO2
If urea is present it will cause the media to become (alkaline) or (acid)? What color?
Alkaline
&
Pink
What color indicates a lack of ability to metabolize urea to ammonia?
No color change
In a Citrate test when ammonium salts (nitrogen source) and citrate (carbon source) are combined it will produce ______________ if citrate is being utilized causing a ____________ media condition.
ammonia
&
alkaline
What color indicates positive results in a Citrate test?
Deep Blue
What color indicates negative results in a Citrate test?
Green
(SIM) media tests for the ability to degrade the ________ __________ __________ that will produce ___________ in the form of ________ ; as a result what color will form?
amino acid cysteine
sulfur
H2S
Black (FeS produced in the media)
What color will be present if the the SIM test is negative?
No black color
SIM media also provides what kind of chemical (also found in Turkey) ?
Tryptophan
Organisms that can degrade __________ will produce indole as a ___________.
Tryptophan
product
In order to detect the presence of indole what is required after incubation?
Kovac’s reagent
What color will Kovac’s reagent turn in the presence of indole?
A RED RING of liquid on top of the medium.
What color will show if the indole test is negative?
A YELLOW RING on top of the tube (reagent had no color change).
