Exam 2 - lab Flashcards Preview

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Flashcards in Exam 2 - lab Deck (26):
1

_____ the inhibitor used against the substrate for alkaline phosphatase

phenyl phosphate

2

_____ the substrate used in alkaline phosphatase lab

p-nitrophenyl phosphate

3

_____ a product of hemoglobin breakdown

bilirubin

heme - biliverdin - bilirubin (free or conjugated)

4

____ the conjugated form of bilirubin

bilirubin glucoronide

5

(T/F) total bilirubin measures both free and conjugated bilirubin

true

6

(T/F) free bilirubin in blood is water soluble and bound to albumin

false

water INSOLUBLE, bound to albumin is true

7

the normal reference range for TOTAL bilirubin
a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2

C

8

the normal reference range for conjugated bilirubin
a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2

D

9

the normal reference range for free bilirubin
a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2

B

10

(T/F) conjugated bilirubin is normally seen circulating in peripheral blood in high concentrations

false

11

the reference range for the condition kernicterus
a. 1-8
b. 6-12
c. 10-14
d. ~20 mg/dl

D

12

the gene in pGLO plasmid that encodes beta-lactamase
a. GFP
b. bla
c. ara
d. pBAD

B

13

_____ is a cluster of structural genes controlled by a promoter

operon

14

____ a sequence of DNA to which RNA polymerase binds to initiate transcription of a gene

promoter

15

(T/F) the presence of arabinose alters the conformation or the protein araC which is bound to pBAD which promotes the transcription of the genes in the operon

true

16

____ the enzyme that converts biliverdin to bilirubin

biliverdin reductase

17

_____ the enzyme that converts heme to biliverdin

hemeoxygenase

18

_____ catalyzes the conjugation of bilirubin in the liver

UDP-glucoronyl transferase

19

_____ this method confirms that a transformed bacteria has taken up a plasmid with the correct DNA sequence

restriction enzyme analysis

20

____ method in harvesting bacteria in minipreps

centrifugation

21

which of the ff is a DNA mini prep volume?
a. 1-5 ml
b. 10 micrograms
c. 100 mg
d. none

B

100mg is a large prep

22

an OD260 reading of 1 corresponds to which of the ff
a. 50 ug/ml ss DNA
b. 10 ug/ml ss oligonucleotides
c. 40 ug/ml ss RNA or DNA
d. 20 ug/ml ds DNA

C

23

an OD260 reading of 1 corresponds to which of the ff
a. 50 ug/ml ds DNA
b. 50 ug/ml ss DNA
c. 40 ug/ml ss RNA or DNA
d. 20 ug/ml ds DNA or RNAw

A

24

____ the ratio that estimates the purity of nucleic acid solution and the values that correstonds to purity

OD260/OD280

1.8 = pure DNA
2.0 pure RNA

25

(T/F) an OD260/OD280 ratio of 1.3 indicates a pure DNA isolation

false

this is DNA contaminated with protein

26

(T/F) an OD260/OD280 ratio of 2.5 indicates a pure DNA isolation

false

this is RNA contaminated with protein