EXPERIMENT 15 HBsAg RAPID TEST

Question 1

size of enveloped Hepa B virus

Answer 1

42 mm

Question 2

genome of hepa B

Answer 2

dsDNA

Question 3

earliest serological marker indicating acute/chronic infection

Answer 3

HBsAg

Question 4

principle of the test

Answer 4

immunochromatography (solid phase “sandwich” immunoassay)

Question 5

2 stages of infection

Answer 5

acute: general flue-like
progressive: hepatomegaly, jaundice, dark urine, ight feces

Question 6

initial lab test

Answer 6

elevation in billirubin and liver enzymes (ALT)

Question 7

diagnosis of hepa B

Answer 7

realtime PCR- sensitive as few as 10 copies per mL
HBV DNA viral load

Question 8

serologic markers

Answer 8

HBsAg- active
HBeAg- infective
IgM anti HBc- current/recent/acute
total anti HBc- current/past
anti HBe- recovery from hepa B
anti HBs- immunity
HBV DNA