extra for Lab test 1 Flashcards
Define Psychrophile? example of bacteria? (temperature)
Like it cold, just blew freezing, up to 20 degrees Celsius
Define Psychrotophs? example of bacteria?(temperature)
0 to 30 degrees Celsius, room temperature, example: pseudomonas fluorescence
Define Mesophile? example of Bacteria?(temperature)
20 to 45 degrees Celsius, moderately warm, example: E. COli
Define thermophile? example of Bacteria?(temperature)
50 to 70 degrees Celsius, like it hot, example: Bacillus stearothermophilus
How to get dilution facter? (standard plate count)
Vol.Added/ total vol
remember 1/10 = .01 = 10^-1
How to calculate number on colony forming unites (CFUs) using the dilution factors?
Colonies x DF = cfu/ml
What colony counts between?
30-300
Dont forget! if more than one plate has countable colonies, take an average.
What was the purpose of transferring the + DNA and - DNA and - DNA tubes from ice, to hot water, to ice again?
Heat- Shock = making competent, to absorb plasmid calcium chloride
Why were the vials incubated for 10 minutes in LB broth rather than transferring their contents directly to the plates?
To transcribe then translate it to make beta lactamase ( break down…) become amphillican resistant
What information is provided by the LB/-DNA plate?
Control plate
- it’ll grow in LB agar
- it’s alive
Oblivious, transformation could occur only if the pGLO plasmid was introduced into the solution. Which plate exhibit transformation?
- because bacteria is growing in the presence of amphiailin
- lb/amp/ara exhibits the transformation
what information is provided by the LB/amp/-DNA plate?
amphicillin kills E. Coli
Why does the LB/am/ara/+DNA plate fluoresce when the LB/amp/+DNA Plate does not?
- LB/amp/ara +DNA fluoresce because pBad was turned on by adding arabimose + araC
- araC is made by bacteria
-LB/amp/+DNA doesnt fluoresce because ara wasn’t present
How can amoebas move?
use of pseudopods
how can paramecium move?
use of cilia
