Lab Quiz 2

Question 1

What do Negative stains do?

Answer 1

Negative stain does not stain organism but stains the backgrounds.

Question 2

how does a capsule appear in prepared slide?

Answer 2

clear surrounded by red stained background

Question 3

Why is negative stained used?

Answer 3

to determin morphology and cellular arrangment in bacterica that are too delicate to withstand heat-fixing. (for example Treponema)

Question 4

What color indicates bacteria is present in the agar? (Motility)

Answer 4

Red(TTC- Help us to visualize the bacterial in the agar)

Question 5

What does the red(TTC) color repersent? (Motility)

Answer 5

bacterial metabolism

Question 6

When oxidized what color is TTC?

Answer 6

colorless

Question 7

When TTC is reduced? What does it mean?

Answer 7

it is red and forms an insoluble precipitate.

wherever you see red the bacterial are there.

Question 8

What does TTC stand for ?

Answer 8

Tetrazolium chloride

Question 9

What are wet mounts used for?

Answer 9

allow obersvation of living cells to determin motility. Also used to see natural cell size, arrangement, and shape

Question 10

What is motility?

Answer 10

Move ones self

Question 11

If it not motility, What is it?

Answer 11

Brownian movement (vibrating)

Question 12

Two type of pigment for slants?

Answer 12

Intracellular( on top agar)

Extacellular (inside agar)

Question 13

Two type of consistency for slants?

Answer 13

Mucoid (smooth and shiny)

Dry ( friable , crusty, and cracky)

Question 14

Four type of growth patterns for broth?

Answer 14

Pelllicule (on top)
Tubridity (cloudy
sediment (at the bottom of the meduim tube)
Flaccutant(clump on side of tube or in agar)

Question 15

What are growth patterns used for?

Answer 15

can provide useful information when attempting to identity an organism.

Question 16

What are common protoza found in lakes and ponds?

Answer 16

Amoeba, Paramecia, Euglena, Rotifers, and Volvox

Question 17

What are the three kinds of locomtion for different protoza?What protoza uses them?

Answer 17

cilia (paramecia)
Flagella (Euglena)
pseudopods (Amoeba)

Question 18

What can Protozoa cause?

Answer 18

Diseasess

Question 19

What does Trypanosoma cause? what class it is in? and What is it pigment?

Answer 19

African sleeping sickness, superclass Mastigophora, and extracellular

Question 20

What does Plasmodium cause? what class it is in? and what is it pigment?

Answer 20

Malaria in humans and animals, superclass sporoza and intracellular.

Question 21

What is the locomotion of Subphylum ciliophora?

Answer 21

ciliates

Question 22

What is the locomotion of Subphylum Sporoza?

Answer 22

apicomplexa

Question 23

what is the locomotion of Subphylum Sarcomastgophora?

Answer 23

Flagellates and amoebas

Question 24

What is the locomotoion of superclass Mastigophora?

Answer 24

Flagellates

Question 25

what is the locomotion of superclass sarcodina?

Answer 25

amoebas

Question 26

What is EMB? What are the colors?

Answer 26

Eosin Methylene Blue Agar Shiney green = E.Coli because love lactose Dark purple = lactose fermented Light purple/Lavander and sometime blue = No lactose fermented the sugars provide fermentable substrates to encourage growth of fecal coliforms

Question 27

What is MAC? What are the colors?

Answer 27

MacConkey Agar Pink = lactose fermentation Not pink= no lactose fermentation Acid accumulating from lactose fermentation turns the dye red Crystal violet allow growth of Enterococcus and some species of Staphylococcus, which ferment the lactose and appear pink on the medium

Question 28

What is MSA? What are the colors?

Answer 28

Mannitol Salt Agar Pink growth doesnt not change color of meduim (staphylococci) (NMF) YELLOW AGAR ( staphlococcus) MF (Mannitol fermented)

Question 29

What is PEA?

Answer 29

Phenylethy Alcohol Agar | allows gorwth of gram postivie organism and stop or inhibit growth of most gram negative organism.

Question 30

What is selective medium?

Answer 30

inhibiting growth of some organisms while encouraging the gorwth of others.

Question 31

What is differential media?

Answer 31

indcators to expose differences between organisms

Question 32

Can particular meduim may be both selective and differental?

Answer 32

YES

Question 33

Describe how a GasPak anaerobic system generates an anaerobic environment?

Answer 33

removing the oxygen by CO2 enriched conditions

Question 34

How can you tell wheather an anaerobic enviroment has been achieved in the jar?

Answer 34

the blue strip is no longer blue

Question 35

Which organism ares facultative anaerobes?

Answer 35

E.coli and B.cereus but E.coli grows better with oxygen

Question 36

which organism are obliagte aerobes?

Answer 36

M. Luteus must need air to grow

Question 37

which organism are obliageted anarobes?

Answer 37

Clostridium Sporogenes can not tolerate air